利用低CO_2浓度培养箱筛选谷子(Setaria italica)C_4光合作用相关突变体

Screening of C_4 Photosynthesis-related Mutants in Foxtail Millet(Setaria italica) by Employmeny of Low CO_2 Concentration Incubator

C_4光合作用和光呼吸研究是植物学界的研究热点,缺乏低CO_2浓度培养条件和相关突变体限制了相关工作的深入开展。本研究设计了一个低CO_2浓度培养箱,CO_2浓度、光照和温度等培养条件可稳定控制,试验数据可通过网络实时查看记录。以该培养箱为平台,本研究对54份谷子甲基磺酸乙酯(EMS)突变体进行了耐40 mg/L(正常空气中CO_2浓度范围是380~400 mg/L)CO_2浓度培养鉴定,根据死苗量将这些材料分为4类,其中敏感的Ⅲ级突变体19个和Ⅳ级突变体13个,对低CO_2浓度极度敏感的5个谷子突变体均为叶脉变异系,证实了低CO_2浓度培养箱筛选鉴定的实用性。对这些材料开展包括花环解剖结构观察和相关突变基因克隆的深入研究,将奠定谷子C_4光合作用的研究基础,所构建的低CO_2浓度培养箱也适用于其他作物光合作用和光呼吸的生理学研究。

C4 photosynthesis and photorespiration studies are research hotspot in plant science. However, lacking low CO2 concentration culture conditions and related mutant resources limit the in-depth development of related work. This study designed a low CO2 concentration incubator which can stably control plant growth conditions including light, CO2 concentrations and temperature. Test data can be simultaneously updated onto the user-friendly remote website that allows to easily check the experimental records. Taking advantage of that, 54 EMS （Ethyl methane sulfonate） induced mutants of foxtail millet were subjected to be tested under 40 mg/L （ concentration u- nits：parts per million. The range of CO2 concentration is 380-400 mg/L in normal air condition）CO2 concen- tration. According to the number of survival seedlings at death point, these mutants can be placed into four groups. 19 mutants at level Ⅲ and 13 mutants at level Ⅳ, were found and recognized to be sensitive to CO2 stress. There are also 5 mutants with vein variations which were hypersensitive to low CO2 concentration. Those results confirmed low concentration of CO2 incubator was applicable in screening for C4 photosynthesis-related mutants, and also use for the in-depth study of kranz anatomical structures observation and relevant gene cloning. In addition, the newly-developed low-concentration CO2 incubator can also be applied for analyzing photosynthesis and respiration in other main crops.