miRNAs在原发性肝癌中的表达及临床意义分析

Expression and Clinical Significance of miRNAs in Primary Carcinoma of Liver

目的:验证miRNAs在肝癌及癌旁组织样本中的表达,并探讨miRNAs表达水平与2年乙肝相关性肝癌复发转移的相关性。方法:采用实时荧光定量逆转录聚合酶链式反应(real-time PCR)检测miR-21、miR-151、miR-96及miR-198在30例肝癌及癌旁组织样本中的表达情况,并分析miRNAs的表达水平与临床病理特征的关系。结果:与癌旁组织相比,miR-21、miR-96及miR-151肝癌组织较癌旁组织中表达上调,占总体样本的76.7%、70%、66.7%;而miR-198在肝癌组织中表达下调,占总体样本的66.7%。通过对临床资料的分析,本研究发现miRNAs在肝癌组织中的表达差异与肝癌患者肿瘤数目、分化程度、TNM分期、门静脉癌栓及2年复发转移等密切相关(P<0.05)。结论:miRNAs在肝癌及癌旁组织样本中的表达水平存在明显差异,并与肝癌的恶性表型显著相关;miR-21、miR-151和miR-96能促进肝癌的侵袭转移,而miR-198能抑制肝癌的侵袭转移;miRNAs的共同作用可影响患者的预后。

Objective： To verify the expression of miRNAs of hepatocellular carcinoma（HCC） and adjacent tissue samples,to investigate the correlation between miRNAs expression and recurrence and metastasis of liver cancer-associated with HBV in 2 years. Methods：To detect the expression of miR-21,miR-151,miR-96 and miR-198 in 30 cases of hepatocellular carcinoma and paracancerous tissue samples by real-time quantitative reverse transcriptase polymerase chain reaction,and analyze the relationship between the expression level of miRNAs and clinical pathological features. Results： Compared with the adjacent tissues,the expression of miR-21,miR-96 and miR-151 in hepatocellular carcinoma tissues was higher than adjacent tissues,accounting for 76. 7%,70%,66. 7% of the overall sample;and the expression of miR-198 in hepatocellular carcinoma tissues was lower than adjacent tissues,accounting for 66. 7% of the overall sample. Through the analysis of clinical data,we found that the expression of miRNAs in HCC tissues was closely related to the number of tumor,differentiation,TNM stage,portal vein tumor thrombus and 2 year recurrence and metastasis（P〈0. 05）. Conclusion： There are obvious differences in the expression level of miRNAs in HCC tissue samples,and was associated with the malignant phenotype of hepatocellular carcinoma; miR-21,miR-151 and miR-96 can promote the invasion and metastasis of HCC,and miR-198 can inhibit the invasion and metastasis of HCC,interaction of miRNAs can affect the prognosis of patients.