维吾尔族不同HER-2表型乳腺癌中Survivin和Caspase-9基因表达的相关性及其意义

Correlation and clinical significance of Survivin and Caspase-9 gene expression in Uygur breast cancer patients with different HER-2 phenotypes

目的检测Survivin和Caspase-9基因在维吾尔族不同HER-2表型乳腺癌患者中的表达,分析两基因的差异及相关性;初步探明两基因在维族乳腺癌发病中可能存在的作用。方法选取初次确诊的维族患者72例为研究对象,HER-2阳性39例(A组),HER-2阴性33例(B组);以维族良性乳腺患者(40例)为对照(C组)。采用免疫组化和real-time RT-PCR分别检测两基因的表达,分析其在各组间表达的差异及两基因的相关性。结果 (1)A组和B组Survivin表达均强于C组。A组较B组Survivin表达增强(P<0.05);而B组也较C组表达增强,但差异无统计学意义(P>0.05)。(2)A组和B组Caspase-9表达均低于C组。real-time RT-PCR结果:A组较B组表达减弱(P<0.05);而B组较C组表达略低,但差异无统计学意义(P>0.05)。免疫组化结果:A组及B组与C组比较差异均无统计学意义(均P>0.05)。(3)两基因在A、B组中表达均呈负相关(分别P<0.01,P<0.05)。结论在新疆维吾尔族妇女HER-2阳性乳腺癌患者中,Survivin表达增强,Caspase-9表达减弱,二者呈负相关。Survivin可能通过抑制Caspase-9,导致维族女性HER-2阳性乳腺癌的发生。

Objective To detect the expression of Survivin and Caspase-9 gene in Uygur breast cancer pa-tients with different HER-2 phenotypes,to find out the difference and association of the two genes and to find outthe potential roles of the two genes breast cancer pathogenesis. Methods We selected 72 Uygur patients diag-nosed as breast cancer initially and they were divided into group A with HER-2 positive（n=39）and group B withHER-2 negative（n=33）. Another 40 Uygur patients with benign breast were involved as the controls. Immunohis-tochemistry and real-time RT-PCR were used to detect the two genes,and analyze the differences and associationof each gene between the groups. Results（1）The expression of Survivin gene in group A and B were higher thanthat in the control group. Further analysis showed that the expression of Survivin gene was enhanced in group Awhen compared with that in group B（P〈0.05）;while even the expression of Survivin gene in group B was higherthan that in the control group but no statistical difference was found（P〉0.05）.（2）The expression of Caspase-9 gene in group A and B were lower than that in the control group. Real-time RT-PCR showed that the expression ofCaspase-9 gene of group A was decreased when compared with that in group B（P〈0.05）;While the expressionof Caspase-9 gene of group B was slightly lower that of the control group but it showed no statistical significance（P〈0.05）. Immunohistochemical results showed there were no statistical differences of expression of Caspase-9 gene in group A and B and control group（all P〉0.05）. The expression of Survivin and Caspase-9 gene was nega-tively associated in group A and B（P〈0.01;P〈0.05）. Conclusions In Uygur patients with HER-2 positivebreast cancer,the expression of Survivin gene is enhanced but that of caspase-9 gene is decreased,and they arenegatively associated. Through inhabiting caspase-9 gene,Survivin gene may potentially lead to the occurrence ofHER-2 positive breast cancer.