沉默lncRNA PVT1对鼻咽癌细胞C666-1增殖、侵袭转移的影响

Effects of lncRNA PVT1 silencing on C666-1 cell proliferation,migration and invasion

目的探讨下调长链非编码RNA PVT1(lncRNA PVT1)对鼻咽癌细胞增殖、侵袭转移的影响。方法采用荧光定量PCR的方法检测PVT1在鼻咽癌细胞中的表达;通过四甲基偶氮唑蓝(MTT)法分析转染siPVT1后对鼻咽癌C666-1细胞生长的影响;用Transwell法评估沉默PVT1对C666-1细胞迁移和侵袭能力的影响;荧光定量RT-PCR和Western blot检测上皮间质转化相关蛋白(E-cadherin、N-cadherin和Vimentin)表达。结果 PVT1在鼻咽癌细胞中的表达显著升高;下调鼻咽癌细胞C666-1中PVT1表达可抑制细胞生长、迁移和侵袭;并且抑制N-cadherin和Vimentin的表达,升高E-cadherin的表达。结论下调ln-c RNA PVT1可以抑制鼻咽癌细胞的生长、迁移和侵袭,逆转鼻咽癌细胞上皮间质转化,提示其可作为鼻咽癌治疗的一个潜在靶点。

ObjectiveTo explore the effects of down-regulated PVT1 on the proliferation,migration and invasion of nasopharyngeal cancer（NPC）C666-1 cells.Methods Expression of PVT1 in NPC cell lines was detected by real-time RT-PCR.The effects of PVT1 silencing on cell proliferation were detected using MTT assay.Transwell assay was performed to assess the effect of down-regulated PVT1 on C666-1 cells migration and invasion.Protein level of N-cadherin,Vimentin and E-cadherin were determined by q RT-PCR and Western blot.Results Expression of PVT1 was significantly increased in seven NPC cell lines.Expression of PVT1 was reduced compared to the control group after the si PVT1 transfection.C666-1 cell proliferation was inhibited as compared with si NC group after transfection.The number of migration and invasion cells were significantly reduced while down-regulated PVT1 expression.E-cadherin expression was upregulated while N-cadherin and Vimentinafter was downregulated after PVT1 silencing.Conclusions Downregulation of PVT1 in C666-1 cells via si PVT1 transfection can inhibit cell growth,migration and invasion,as well as reverse epithelial-mesenchymal transition.PVT1 may serve as a biomarker or even a therapeutic target for NPC.