核酸杂交检测在细菌性阴道病及外阴阴道假丝酵母菌病诊断中的应用价值

The application value of DNA hybridization probe test in the diagnosis of bacterial vaginosis and vulvovaginal candidiasis

目的评估核酸杂交法在检测和识别细菌性阴道病、外阴阴道假丝酵母菌病相关微生物的灵敏性和特异性,评估其临床应用价值。方法对门诊具有阴道炎症状或体征的103例患者阴道分泌物进行检测,以Nungent评分、真菌培养为金标准,BV蓝试剂检测法、湿片镜检为常用检测方法,比较核酸检测法的灵敏性、特异性及检测的一致性。结果与Nungent评分相比,核酸检测法的敏感性为98%,特异性为96.2%,高于湿片镜检和BV蓝试剂检测法;与真菌培养相比,核酸杂交法检测的敏感性为89.4%,特异性为98.3%,核酸杂交法与金标准检测方法比较具有较好的一致性。对混合感染的检出准确性高于镜检(83.3%vs.70.8%)。结论与传统的检测方法比较,核酸杂交检测具有较高的敏感度和特异性,可以同时区分和识别细菌性阴道病、外阴阴道假丝酵母菌病和滴虫性阴道炎的致病微生物,尤其在细菌性阴道病的诊断方面优势明显。

Objective To investigate the sensitivity and specificity of DNA hybridization probe test（Af-firm VPIII） in the identification related microorganisms of bacterial vaginosis（BV）,vulvovaginal candidiasis（VVC）. Methods Vaginal secretion were detected in 103 patients with symptom and sign of vaginitis in the outpatient department. According to Nungent score,the fungal culture as the gold standard. BV blue reagent assayand wet mount microscopy as a common detection method,evaluated the sensitivity and specificity of AffirmVPIII. Results Compared with the Nungent score,the sensitivity and specificity of Affirm VPIII were 98% and96.2%. Compared with the fungi culture,the sensitivity and specificity of Affirm VPIII were 89.4% and 98.3%.Conclusion Affirm VPIII has high sensitivity and specificity which can identify pathogenic microorganisms of BV,VVC,especially in BV diagnosis.