miR-655和神经胶质瘤临床特征的关系及其在诊断和预后中的作用

Relationship between miR-655 with the clinical features of glioma and its role in diagnosis and prognosis

目的探讨miR-655和神经胶质瘤临床特征的关系及其在诊断和预后中的作用。方法收集2013年1月至2017年5月在内蒙古医科大学附属医院进行手术的97例胶质瘤患者的组织和癌旁组织,采用实时荧光定量PCR检测miR-655的表达,分析miR-655在神经胶质瘤的诊断和预后中的作用。结果和神经胶质瘤癌旁组织相比,癌组织中miR-655的表达显著降低(P<0.001),其表达水平与肿瘤大小(P=0.024)、WHO分级(P=0.002)显著相关。COX回归分析表明miR-655的表达(P=0.004)和肿瘤切除程度(P=0.008)是神经胶质瘤的独立危险因素;ROC分析表明miR-655诊断神经胶质瘤的曲线下面积(AUC)为0.959(95%CI:0.936~0.982),当miR-655表达为0.755时其诊断的敏感度和特异度分别为89.7%和88.7%。Kaplan-Meier分析神经胶质瘤的表达<0.426组和≥0.426组之间生存时间差异有统计学意义(P=0.004),miR-655的表达越低说明神经胶质瘤患者的预后越差,miR-655是影响神经胶质瘤患者生存的独立的危险因素。结论 miR-655能够作为诊断和预后不良预测的标志物。

Objective To study the relationship between miR-655 with the clinical features of glioma and its role in diagnosis and prognosis.Methods The tissues and para cancerous tissues of 97 patients with glioma were collected from January 2013 to May 2017 at the Affiliated Hospital of Inner Mongolia Medical University.The expression of miR 655 was detected by real-time fluorescence quantitative PCR,and the diagnosis and prognosis of miR-655 in glioma were analyzed.Results The expression of miR-655 in the cancerous tissues was significantly lower than that of the paracancerous tissues of the glioma（P0.001）,which was significantly related to the size of the tumor（P=0.024）and the WHO grading（P=0.002）.COX regression analysis showed that the expression of miR-655（P=0.004）and the degree of tumor resection（P=0.008）were independent risk factors for glioma;ROC analysis showed that the area under the curve of the glioma（AUC）under the miR-655 expression was 0.959（95%CI：0.936-0.982）;when the expression of miR-655 was 0.755,the sensitivity and specificity of the diagnosis were 89.7% and 88.7% respectively.The Kaplan Meier analysis of the expression of glioma was statistically significant（P=0.004）.The lower expression of miR-655 indicated the worse the prognosis of glioma patients,the COX analysis miR-655 was an independent risk factor for the survival of glioma patients.Conclusion miR-655 can be used as a marker of poor prognosis in diagnosis and prognosis.