氯喹对MCF7细胞增殖的抑制作用及其机制

Inhibition of chlorouine on MCF7 cell proliferation and its mechanism

目的:探讨氯喹对乳腺癌细胞MCF7的生长抑制作用及其可能机制。方法:取对数生长期的MCF7细胞,用不同浓度氯喹(0、10、20、30、40μmol/L)作用于MCF7细胞不同时间(0 h、24 h、48 h和72 h),通过MTT法检测细胞增殖情况。使用显微镜观察不同浓度氯喹作用MCF7细胞72 h后细胞形态变化。利用Western blot方法检测不同浓度氯喹作用MCF7细胞24 h后细胞内β-catenin蛋白和Cyclin D1蛋白变化情况。结果:与对照组比较,氯喹在一定浓度范围内对MCF7细胞有明显的抑制作用(P<0.05),细胞增殖活性随着剂量和时间增加而逐渐下降。显微镜下观察到氯喹处理MCF7细胞后,细胞数目减少而细胞碎片增加。Western blot结果显示氯喹处理MCF7细胞后,细胞内β-catenin和Cyclin D1蛋白表达出现下调(P<0.05)。结论:氯喹对MCF7细胞增殖具有抑制作用,其机制可能与下调Wnt/β-catenin信号通路有关。

Objective： To investigate the effect of chlorouine on breast cancer cell line MCF7 and its potential mechanism. Methods： MCF7 cells were treated with chlorouine in various concentrations（0,10,20,30,40 μmol/L） for different durations（0 h,24 h,48 h and 72 h）.The cells viability was determined by MTT method.The morphological changes of cells incubated with chlorouine after 72 h were observed under the microscope.The expression of β-catenin protein and Cyclin D1 protein was assayed by western blot after MCF7 cells were treated with chlorouine in various concentrations for 24 h. Results： Compared with control group,MCF7 cells proliferation was inhibited in a time and concentration dependent manner when treated with chlorouine（ P 〈0.05）.The number of cells decreased while cell fragments increased with chlorouine concentration increased.The expression of β-catenin and Cyclin D1was both significantly down-regulated after treatment of chlorouine（ P 〈0.05）. Conclusion： Chlorouine could inhibit MCF7 cells proliferation and the mechanism may be involved in Wnt/β-catenin pathway.