C57BL/6小鼠尾静脉四种穿刺方法的比较

Comparison of different puncture methods in C57BL/6 mouse tail veins

目的探究C57BL/6小鼠不同尾静脉注射方法的差异,为小鼠尾静脉注射提供更为安全、可靠、成功率高的基础实验依据。方法将320只小鼠按照随机数字法随机分成4组:空白对照组、白炽灯烘烤法组、三头线法组和联合应用法组。选择穿刺小鼠左侧或右侧外周静脉进行取血,记录各组穿刺成功率,采用SPSS 13.0统计软件进行分析,比较各组间的统计学差异。结果与空白对照组相比,白炽灯烘烤法组、三头线法组和联合应用法组穿刺成功率均显著提高(P<0.001)。其中,三头线法组优于白炽灯烘烤法组(P<0.001),而联合应用法组成功率则明显高于三头线法组和白炽灯烘烤法组(P<0.001)。结论 C57BL/6小鼠尾静脉不同注射方法中,联合应用法操作简单,可重复性高,穿刺成功率很高,值得进一步推广使用。

Objective Four different methods were examined to identify a safer and more reliable method for tail vein punctures in C57 BL/6 mice. Methods In total,320 mice were randomly divided into four groups： a blank group,incandescent lamp baking method group,three-line method group,and combined method group. Blood samples were taken from the left or right peripheral vein of puncture mice. Puncture success rate of each group was recorded. SPSS 13. 0 software was used to compare statistical difference among groups. Results Compared with the blank group,success rates of the other three methods were significantly higher（P〈0. 001）. Further,the three-line method was better than the incandescent lamp baking method（P〈0. 001）. The success rate of the combined method was significantly higher than the three-line and incandescent lamp baking methods（P〈0. 001）. Conclusions The combined method greatly improved the success rate of tail vein punctures in C57 BL/6 mice. This method is more reliable and should be more widely used in the future.