载玻片加热在冰冻切片帖片中的运用

Application of heating microscope slide in frozen section

目的:探讨载玻片加热在冰冻切片帖片中的运用效果。方法:对100例行快速准确术患者的共计200份标本进行分组,采取的分组方法为随机数字表法,其中对对照组应用传统的、规范的快速冰冻方法制片,对观察组应用载玻片加热法制片,对比两组标本染色后的效果。结果:观察组脱片率为1.0%(1/100),对照组脱片率为8.0%(8/100),观察组切片质量明显优于对照组,经过显著性检验,P<0.05。结论:在规范规范的快速冷冻方法制片基础上,采用载玻片加热法进行快速冰冻切片能够有效的降低组织脱片的发生概率,且细胞组织形态完整,背景清晰,制片时间短,值得推广使用。

Objective： To explore the effect of heating microscope slides on frozen section. Methods： 200 sample from 100 patients were selected and grouped. The control group took the traditional norms of fast freezing method, and the observation group applied heating microscope slides. Results： Dropping rate is 1.0% in the observation group, better than 8.0% in the control group, P〈0.05. Conclusion： Heating microscope slides can effectively reduce the dropping, with complete cell structure, clear background and short time of production.