代谢解偶联剂四氯水杨酰苯胺的污泥减量性能和机理研究

Mechanism and performance of metabolic uncoupler tetrachlorosalicylanilide in sludge reduction

为解析代谢解偶联剂的污泥减量性能及机理,选用毒副作用较低的代谢解偶联剂四氯水杨酰苯胺(TCS),考察其对序批式活性污泥反应器(SBR)长期运行过程中污泥产量及运行参数的影响。结果表明,投加一定量的TCS具有较好的污泥减量化作用,添加TCS前和停用后,SBR内各指标变化均不明显,说明TCS对SBR运行没有明显影响。当SBR中污泥混合液悬浮固体(MLSS)为2 200mg/L,TCS添加量为1.6mg/L时,平均污泥产率系数由0.521mg/mg降至0.314mg/mg,污泥产量减少39.73%。TCS对有机物的去除基本没有影响,COD去除率仅下降3.03百分点,但比耗氧速率(SOUR)增加73.73%,比三磷酸腺苷(SATP)合成量减少23.90%,胞内贮存物(PHAs)含量平均增加42.28%,脱氧核糖核酸(DNA)含量无明显变化。因此,适量添加TCS不会造成细胞溶胞,但能使胞内代谢增加,使氧化磷酸化解偶联,使生物合成量减少,从而实现污泥减量化。

In order to analyze the mechanism of sludge reduction by chemical metabolic uncoupler,the chemical uncoupling tetrachlorosalicylanilide（TCS）with little the poisonous side effect was selected to investigate its effect on sludge reduction during long-term operation of sequencing batch reactor activated sludge process（SBR）.Results showed that before and after the addition of TCS,the operating index of SBR changed little,indicating that TCS had no significant effect on SBR in long-term operation.The proper uncoupler could play an important role in sludge reduction.At an TCS concentration of 1.6 mg/L,the average sludge yield coefficient decreased from 0.521 mg/mg to0.314 mg/mg,which supposed sludge reduction of 39.73%to SBR system（MLSS was 2 200 mg/L）.TCS had little effect on the removal of organic matter,the COD removal rate decreased only 3.03 percent point.Specific oxygen uptake rate（SOUR）increased sharply by 73.73%,specific triphosphate（SATP）synthesis quantity was declined about 23.90%.The intracellular storage（PHAs）content increased with an average rate of 42.28%.There was no significant difference on DNA quantity in test and control group.Appropriate dosage of TCS would not cause cell lysis and maintain an increase in intracellular metabolism,uncoupling oxidative phosphorylation would reduce the amount of biosynthesis,thus contributing to reduce sludge yield.