铁离子对幽门螺杆菌感染的胃上皮细胞LncRNA的影响

The effect of iron ion on LncRNA of gastric epithelial cells with Helicobacter pylori infection

目的探讨铁离子对幽门螺杆菌(Hp)感染的胃上皮细胞(GES-1)长非编码RNA(LncRNA)的影响。方法以胃上皮细胞作为对照A组,Hp感染胃上皮细胞作为B组,硫酸铁与胃上皮细胞共培养为C组,硫酸铁与Hp和胃上皮细胞共培养为D组,采用表达谱基因芯片检测各组GES-1细胞的LncRNA和mRNA基因差异表达。结果与A组LncRNA基因数相比:B组上调76个LncRNA基因,下调45个基因;C组上调40个基因,下调123个基因;D组上调124个基因,下调159个基因。各组间差异基因主成分分析显示B组比C组和D组的离散程度高。在功能富集分析显示,TNF信号通路中,C组和D组与B组比较,相关mRNA基因多数下调。结论铁离子可能影响胃上皮细胞LncRNA调控作用进而影响Hp感染的发病情况。

Objective To investigate the effect of iron ion on LncRNA of gastric epithelial cells（GES-1）with Helicobacter pylori（Hp）infection. Methods Group A was gastric epithelial cells as controls,group B was gastric epithelial cells with Hp infection,group C was ferric sulfate with gastric epithelial cells（cultured together）,group D was gastric epithelial cells induced by ferric sulfate after infected by Hp（cultured epithelial cells,ferric sulfate and Hp together）.The differential expressions profile of LncRNA and mRNA were detected by Microarray. Results Comparison of LncRNA gene number of group A with the other groups showed：76 LncRNA genes were up-regulated and 45 genes were down-regulated in group B;40 genes were up-regulated and 123 genes were down-regulated in group C;124 genes were up-regulated and 159 genes were down-regulated in group D.The main components of the difference genes in each group were analyzed and results showed that the dispersion degree of group B was higher than those of group C and group D.The function enrichment analysis of TNF signaling pathway showed that the expression of associated mRNA genes in group C and group D were mostly down-regulated compared with group B. Conclusion Iron ion may affect the regulation of LncRNA in gastric epithelial cells and then affect the incidence of Hp infection.