空气霉菌总数测定方法分析

Measurement Analysis of Fungal Counts in Air

目的择优选择稳定性较高的空气霉菌总数测定方法。方法在不同采样点和采样时间下,同时用自然沉降法和撞击法进行空气霉菌采样,每组三个平行样品,平板计数结果经格鲁布斯检验,剔除离群值,再用平均值计算出每平方米的空气霉菌总数。对比两种采样方法平板计数结果的变异系数,以及采样时间对计算结果的影响。结果撞击法采集的平行样品变异系数小,并且在15 min的采样时间内,受采样时间的影响较小。结论撞击法测定空气霉菌总数稳定性更好。

Objective To look for more stable method for measuring fungal counts in air. Methods In different sampling points and time, the natural precipitation method and impacting method were used to gather fungus in air at the same time. There were three parallel samples in each treatment group. Fungal counts that cultured in plates were tested by Grubbs’ test method and outliers were eliminated. Fungal counts of air were calculated based on the means of parallel samples. Variation coefficient of two measurements’ fungal counts that cultured in plates and influences of sampling time on the calculation result were compared. Results Variation coefficient of impacting method was smaller, and sampling time had less influence on impacting method within 15 minutes. Conclusion The impacting method is more stable to measure fungal counts of air.