抗核抗体与EB病毒抗体水平的关系

Relationship between the levels of anti-nuclear antibodies and Epstein-Barr virus antibodies

目的探讨EB病毒(EBV)特异性抗体对抗核抗体(ANA)阳性患者和ANA阴性健康对照者的免疫反应。方法采用间接免疫荧光法检测ANA,采用免疫印迹法检测抗可提取性核抗原(ENA)抗体,采用酶联免疫吸附试验(ELISA)检测80例ANA阳性患者和48名体检健康者血清EBV-衣壳抗原(VCA)Ig M、EBV-VCA Ig G、EBV-早期抗原(EA)Ig G和EBV核抗原(EBNA-1)Ig G水平,比较ANA阳性患者与ANA阴性健康对照者的EBV抗体阳性率、EBV抗体水平差异,分析ANA阳性患者EBV抗体水平与ENA抗体种类、个数之间的关系。结果 ANA阳性组血清EBV-VCA Ig G、EBV-EA Ig G和EBNA-1 Ig G水平较健康对照组明显升高(P<0.01),且EBV-EA Ig G阳性率明显高于健康对照组(χ~2=5.747,P=0.017)。抗ENA抗体谱中,抗SS-A/Ro抗体、抗SS-B/La抗体阳性组分别与抗SS-A/Ro抗体、抗SS-B/La抗体阴性组比较EBNA-1 Ig G水平明显升高(Z=-2.630,P=0.008;Z=-2.515,P=0.011),EBV-VCA Ig G和EBV-EA Ig G水平差异无统计学意义(P>0.05)。抗核小体抗体阳性组与抗核小体抗体阴性组比较EBV-VCA Ig G、EBV-EA Ig G水平明显升高(Z=-2.355,P=0.018;Z=-2.017,P=0.043),但2组之间EBNA-1 Ig G水平差异无统计学意义(P>0.05)。抗ENA抗体谱中自身抗体阳性个数不同的ANA阳性患者EBV-EA Ig G水平不同(χ~2=11.380,P=0.023)。结论EBV复发感染在ANA的产生中起重要作用。

ObjectiveTo evaluate the specific antibody immune response against Epstein-Barr virus （EBV）in patients with anti-nuclear antibody （ANA） positivity and negativity. MethodsANA was determined by indirectimmunofluorescence. Anti-extractable nuclear antigen （ENA） antibodies were determined by immunoblotting.Serum EBV-virus capsid antigen（VCA） IgM,EBV-VCA IgG,EBV-early antigen（EA） IgG and EBV nuclearantigen（EBNA-1） IgG levels were determined by enzyme-linked immunosorbent assay（ELISA） in 80 patientswith ANA positivity and 48 healthy subjects. The difference of EBV antibody positive rates and levels was evaluatedin ANA-positive patients in comparison with those in ANA-negative healthy controls. The relationship between EBVantibody level and the types and numbers of ENA antibodies was analyzed. ResultsEBV-VCA IgG,EBV-EA IgGand EBNA-1 IgG levels were higher in ANA-positive patients than those in healthy controls （P〈0.01）,and ANApositivepatients showed a higher EBV-EA IgG positive rate compared to healthy controls（χ2=5.747,P=0.017）.Furthermore,elevated EBNA-1 IgG was found in anti-SS-A/Ro antibody positive and anti-SS-B/La antibodypositive groups compared to those in anti-SS-A/Ro antibody negative and anti-SS-B/La antibody negative groups（Z=-2.630,P=0.008;Z=-2.515,P=0.011）. EBV-VCA IgG and EBV-EA IgG levels had no statistical significance（P〉0.05）. EBV-VCA IgG and EBV-EA IgG were higher in anti-nucleosome antibody positive group than thosein anti-nucleosome antibody negative group （Z=-2.355,P=0.018;Z=-2.017,P=0.043）,while there was nostatistical significance for EBNA-1 IgG （P〉0.05）. There was difference in the number of anti-ENA antibodies andthe distribution of EBV-EA IgG levels （χ2=11.380,P=0.023）. ConclusionsEBV reactivation might be correlatedwith the presence of ANA.