FBP1在肾透明细胞癌组织中的表达变化及意义

FBP1 expression change in renal clear cell carcinoma tissue and its significance

目的探讨果糖1,6二磷酸酶1(FBP1)在人肾透明细胞癌(RCCC)及其癌旁组织中的表达,以及在肾癌发生、发展及预后中的作用和临床意义。方法选取手术切除的118例RCCC患者的石蜡切片和40例RCCC患者的新鲜标本,分别采用免疫组织化学方法、Western blot和逆转录聚合酶链反应(RT-PCR)方法检测肾癌及对应癌旁组织(阴性切缘)中FBP1蛋白及mRNA的表达,分析其与患者临床病理特征及预后的相关性。结果免疫组织化学发现78.81%(93/118)的癌旁组织中可见FBP1蛋白呈强阳性表达,仅39.83%(47/118)的肾癌组织中FBP1蛋白表达阳性。Western blot发现肾癌组织中FBP1的表达阳性率较其相应癌旁组织明显下降(P<0.01)。RT-PCR法发现癌旁组织FBP1 mRNA表达水平亦明显高于肾癌组织(P<0.05)。FBP1低表达与临床分期、病理分级、危险系数及是否复发明显相关(P<0.05),与年龄、性别、症状及肿瘤大小、部位、是否有坏死、是否有血管侵犯和肾上腺是否有累及无关(P>0.05)。FBP1阳性表达的肾癌患者的5年生存率高于FBP1阴性表达者(P<0.05)。结论 FBP1及其蛋白在人肾癌组织中低表达,与肾癌的发生、发展相关,可能成为肾癌预后的侯选标志物之一。

Objective To investigate the expression of FBP1 in human renal clear cell carcinoma and paracancerous tissue and its effect and clinical significance in the carcinogenesis,progression and prognosis of renal cancer.Methods The paraffin sections from 118 patients with renal clear cell carcinoma treated by surgical resection and fresh specimens from 40 patients with renal clear cell carcinoma were selected.The expressions of FBP1 protein and mRNA in renal cancer and paracancerous tissues（negative incisal edge）were detected by adopting the immunohistochemistry（IHC）,Western blot and RT-PCR.Its correlation with clinicopathologic characteristics and prognosis of the patients was analyzed.Results The IHC result,found that strong positive expression of FBP1 protein could be seen in 78.81%（93/118）of cancer-adjacent tissues,while only 39.83%（47/118）of renal cancer tissues had positive expression.Western blot found that the expression positive rate of FBP1 in renal cancer tissue was significantly decreased compared with corresponding cancer-adjacent tissues（P〈0.01）.RT-PCR found that the FBP1 mRAN expression level in cancer-adjacent tissues was also significantly higher than that in renal cancer tissue（P〈0.05）.The FBP1 low expression was significantly correlated with the clinical stage,pathologic grade,UISS risk coefficient and recurrence（P〈0.05）,and had no relation with the age,gender,symptoms,tumor size,location,tumor necrosis,vascular invasion and adrenal involvement（P〉0.05）.The 5-year survival rate in renal cancer patients with FBP1 positive expression was higher than that in the patients with FBP1 negative expression（P〈0.05）.Conclusion FBP1 and protein are lowly expressed in renal cancer tissue,are correlated with occurrence and development of renal cancer,and may become one of candidate markers of renal cancer prognosis.