IL-1β、IL-6及下游IDO激活与大鼠抑郁行为的关系研究

Relationship between activation of IL-1β,IL-6 and downstream IDO activation with rat depression behaviors

目的研究慢性不可预见刺激(CUS)致大鼠抑郁行为与炎症因子白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)蛋白表达及吲哚胺2,3-双加氧酶(IDO)mRNA及蛋白表达的关系。方法将30只雄性SD大鼠分为对照组(CG组)和模型组(MG组),采用孤养结合CUS连续刺激28d诱导建立大鼠抑郁症模型。采用开场实验及强迫游泳实验评价大鼠的抑郁行为,qRT-PCR测定海马和皮层IDO mRNA表达;Western blot法测定海马和皮层IL-1β、IL-6及IDO蛋白表达。结果与CG组相比,MG组大鼠开场实验得分明显降低(P<0.01),强迫游泳实验不动时间明显延长(P<0.01),海马和皮层的IDO mRNA表达均明显升高(P<0.01),海马和皮层的IL-1β、IL-6及IDO蛋白表达均明显升高(P<0.05)。结论 CUS致大鼠抑郁行为可能与脑内炎症因子表达增加并诱导IDO过表达相关。

Objective To investigate the relationship between the rat depression behaviors induced by chronically unpredicted stress （CUS） with interleukin-1β （IL-1β）,interleukin-6 （IL-6） protein expression and indole-2,3-dioxygenase （IDO） mRNA and protein expression. Methods Thirty male Sprague Dawley （SD） rats were divided into the control group （CG） and model group （MG）.The solitary raise combined with CUS 28 d continued stimulus was used to establish the rat depression model.The open field test （OFT） and the force swimming test （FST） were used to evaluate the rat depression behaviors;qRT-PCR was used to determine the mRNA expression of IDO in hippocampus and cortex;Western blot was used to determine the protein expressions of IL-1β,IL-6 and IDO in hippocampus and cortex. Results Compared with the CG group,the OFT score in the rats of the MG group was significantly decreased（ P 〈0.01）,the immobility time in FST was significantly extended （ P 〈0.00）,the expression of IDO mRNA in hippocampus and cortex was significantly increased （ P 〈0.01） and the protein expression of IL-1β,IL-6 and IDO in hippocampus and cortex was significantly increased （ P 〈0.05）. Conclusion The rat depression behaviors induced by CUS may be associated with the increase of intracerebral inflammatory cytokine expression and inducing IDO over-expression.