丹参酮ⅡA磺酸钠对高温环境下力竭运动后骨骼肌的保护作用研究

Protective effect of tanshinoneⅡ A sodium sulfonate on skeletal muscle after exhaustive exercise in high temperature environment

目的分析丹参酮ⅡA磺酸钠对高温环境下力竭运动后大鼠骨骼肌的保护作用。方法将90只大鼠分为观察组、空白组、对照组,每组30只。空白组大鼠不接受任何干预处理;观察组和对照组大鼠在高温环境下力竭运动干预,对照组大鼠在高温环境下力竭运动处理后5h经股静脉注射生理盐水8mL/kg,观察组经股静脉注射丹参酮ⅡA磺酸钠40mg/kg。各组大鼠处死前经尾动脉采集动脉血,同时将大鼠右腿腓肠肌取下冻存。检测大鼠血清肌酸激酶(CK)、乳酸脱氢酶(LDH)活性,TNF-α、IL-6、IL-1β水平,以及骨骼肌中丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX)水平;观察各组大鼠骨骼肌标本进行切片HE染色后的形态学变化。结果观察组大鼠血清TNF-α、IL-6、IL-1β水平和CK、LDH活性均明显低于对照组,高于空白组,差异有统计学意义(P<0.05)。观察组大鼠骨骼肌MDA、SOD、GSH-PX水平与其他两组比较差异有统计学意义(P<0.05)。对照组大鼠骨骼肌结构紊乱,肌细胞核分布不均匀;空白组大鼠骨骼肌结构正常,肌细胞核分布均匀;观察组大鼠骨骼肌形态结构正常,但是肌细胞核分布稍显不均匀。结论丹参酮ⅡA磺酸钠可以有效保护在高温环境下力竭运动后的骨骼肌。

Objective To analyze the protective effect of tanshinone Ⅱ A sodium sulfonate on rat skeletal muscle after exhaustive exercise in high temperature environment. Methods Ninety rats were divided into the observation group,blank group and control group,30 cases in each group.The rats in the blank group received no intervention,while which in the observation group and control group received the exhaustive exercise intervention under high temperature environment.The rate in the control group was injected with 8 mg/kg normal saline by femoral vein at 5 h after exhaustive exercise under high temperature environment,while which in the observation group was injected with 40 mg/kg tanshinone Ⅱ A sodium sulfonate by femoral vein.The arterial blood in each group was collected by tail vein before killing the rat,and the right leg gastrocnemius muscle of each group was removed and frozen.The serum CK,LDH activity,TNF- alpha,IL-6,IL-1β,MDA,SOD and GSH-PX levels in skeletal muscles were examined.The rat skeletal muscle samples were conducted the HE staining for observing the morphological change. Results The levels of serum TNF-α,IL-6 and IL-1β and activity of CK and LDH in the observation group were significantly lower than those in the control group,while higher than those in the blank group,the difference was statistically significant （ P 〈0.05）.The skeletal muscle MDA,SOD and GSH-PX levels had statistical difference between the observation group and other two groups （ P 〈0.05）.The skeletal muscle structure was disordered,and the distribution of muscle nuclei was uneven.The structure of rat skeletal muscle in the blank group was normal,and the muscle nuclei were evenly distributed.The morphology and structure of skeletal muscle in the observation group were normal,but the distribution of muscle nuclei was slightly uneven. Conclusion Tanshinone Ⅱ A sodium sulfonate can effectively protect skeletal muscle after exhaustive exercise inhigh temperature environment.