QHF复方对人肝癌Huh7细胞生长、侵袭及肿瘤干细胞表面标志物表达的影响

Effect of Chinese herbal compound QHF on human Huh7 hepatoma cell proliferation and invasion, and expression of surface markers of cancer stem cells

目的:研究中药QHF复方对人肝癌Huh7细胞生长、凋亡和侵袭能力的作用,并初步研究其对肿瘤干细胞表面标志物表达的影响。方法:体外培养人肝癌Huh7细胞,MTT法检测QHF复方对细胞增殖的影响;流式细胞术检测QHF复方对Huh7细胞周期及凋亡的影响;Transwell实验检测QHF复方对Huh7细胞侵袭能力的影响;流式细胞仪检测QHF复方作用后Huh7细胞肿瘤干细胞表面标志物CD133和CD44的表达情况。结果:与溶剂对照组比较,QHF复方能够抑制Huh7细胞增殖,并且这种抑制作用具有剂量和时间依赖性(P均<0.05);QHF复方能诱导细胞凋亡,使其生长周期阻滞在S期;QHF复方能够降低Huh7细胞的侵袭能力(P<0.05);并能够下调肿瘤干细胞表面标记物CD133和CD44的表达(P<0.05)。结论:中药QHF复方能够抑制Huh7细胞的生长、侵袭,这种抑制作用可能与肿瘤干细胞表面标记物CD133和CD44表达的下调有关。

OBJECTIVE：To explore the influence of Chinese herbal compound QHF on human Huh7 hepatoma cell proliferation,apoptosis and invasion and expression of surface markers of cancer stem cells.METHODS：Human Huh7 hepatoma cells were cultured in vitro.Cell proliferation inhibition rate was detected by MTT assay;cell cycle,apoptosis and cell surface marker CD133 and CD44 expression were detected by Flow cytometry;transwell experiment was used to observe cell invasion.RESULTS：QHF inhibited the proliferation of Huh7 cells,and there was a dosedependent inhibitory effect.In addition,QHF promoted apoptosis and significantly increased cell proportion in S phase of the cell cycle.QHF lowered expression of tumor stem cell surface markers：CD133 and CD44.CONCLUSION：QHF was able to inhibit human Huh7 hepatoma cell proliferation and migration in vitro,the inhibition was related to the reduced expression of tumor stem cell surface markers：CD133 and CD44.