精子与肿瘤细胞融合模型的生物学特性研究

Biological characteristics of a sperm-tumor cell fusion model

目的:探讨精子与肿瘤细胞融合模型的生物学特性。方法:采用体外共培养对精子与肿瘤细胞融合模型的时效性、细胞接种密度依赖性及细胞类型特异性进行分析。采用显微操作及单克隆培养法获得小鼠精子与HeLa细胞融合的嵌合型单克隆细胞株,并对其进行生物学鉴定。采用体外扩增Y染色体特异基因TSPY的方法鉴别宫颈癌石蜡组织中的嵌合型肿瘤细胞。结果:精子与肿瘤细胞在体外的融合通常发生于共培养后的1.5 h,精子与肿瘤细胞融合率随细胞接种密度的升高而降低,精子与腺癌来源的肿瘤细胞具有更高的细胞融合率。在20例宫颈癌蜡块标本中我们发现1例存在嵌合型肿瘤细胞,而且其TSPY基因存在1处有义突变。结论:精子与肿瘤细胞融合存在时效性、细胞接种密度依赖性及细胞类型特异性特征。在宫颈癌标本中存在嵌合型肿瘤细胞。

OBJECTIVE：To explore the biological characteristics of sperm-tumor cell fusion model.METHODS：A co-cultivation in vitro methods was used to study the time-effectiveness,cell inoculation density dependence and cell type specificity of the fusion model.The chimeric sperm-HeLa monoclonal cells were obtained by using micromanipulation and monoclonal culture method,which was then identified by using PCR test.The chimeric tumor cells in the cervical cancer paraffin were detected by amplification Y chromosome specific gene TSPY.RESULTS：Fusion between the sperm and tumor cells generally occurred in 1.5 hours after co-cultivation,the fusion rates was inversely proportional to the cell inoculation density,and the tumor cells who derived from an adenocarcinoma had a higher rate of cell fusion than other tumor cells and normal controls.One chimeric tumor tissue was observed in 20 cases of cervical cancer specimens,and one righteous mutation was found in TSPY gene.CONCLUSION：Cell fusion between the sperm and tumor cells displayed time-effectiveness,cell inoculation density dependence and cell type specificity.The chimeric tumor cells could be found in cervical cancer specimens.