摘要
目的:探讨不同剂量邻苯二甲酸二(2-乙基)己酯(DEHP)染毒后硒对大鼠外周血血细胞参数的影响。方法:将77只健康清洁级雄性SD大鼠,随机分为7组,即对照组、3个不同浓度(300、600、900 mg/kg)DEHP染毒组、1mg/kgSe+不同剂量(300、600和900 mg/kg)DEHP染毒干预组,连续饲喂90d,试验期间观察大鼠中毒状况(精神亢奋、被毛蓬松、局部脱毛现象),试验期末称取大鼠体质量并取全血测定各类血细胞参数的变化。结果:与对照组比较,900 mg/kg DEHP染毒大鼠体质量明显下降(P<0.05),600和900 mg/kg DEHP染毒组大鼠出现中毒体征比例分别为63.63%(7/11)和100%(11/11),均较对照组0(0/11)显著升高(P均<0.05)。与相同剂量DEHP染毒组比较,1mg/kgSe干预后大鼠体质量均明显增加,差异均有统计学意义(P均<0.05);300、600mg/kg DEHP染毒组大鼠中毒体征明显降低(P均<0.05)。白细胞参数中,与对照组比较,600和900 mg/kg DEHP染毒组大鼠产白细胞数(WBC)明显降低、单核细胞(MONO)显著升高,差异均有统计学意义(P均<0.05)。与相同剂量DEHP染毒组比较,1mg/kgSe+600mg/kg DEHP干预组白细胞数(WBC)明显升高、单核细胞(MONO)显著降低(P<0.05)。红细胞参数中,与对照组比较,DEHP染毒后大鼠平均血红蛋白量(MCH)、血红蛋白含量分布宽度(HDW)均明显升高;600、900mg/kg DEHP染毒组大鼠红细胞数(RBC)、红细胞压积(HCT)明显降低(P<0.05)。与相同剂量DEHP染毒组比较,1mg/kg Se干预后大鼠红细胞参数未见显著差异。血小板参数中,未发现DEHP染毒与Se干预对SD大鼠血小板各参数产生显著影响。结论:300~900 mg/kg DEHP染毒,可对大鼠某些红细胞和白细胞参数产生影响,硒的干预在300~600 mg/kg DEHP染毒条件下可保护某些血细胞功能。
OBJECTIVE:To investigate the effects of different doses of di (2-ethylhexyl) phthalate (DEHP) on blood cell parameters in rats after 1 mg/kg selenium's intervention.METHODS:Seventy-seven healthy male SD rats were randomly divided into 7 groups:the control group,three DEHP groups with different concentrations (300,600,900 mg/kg),and Se +different doses of DEHP (1 mg/kg Se + 300,600,and 900 mg/kg DEHP).The rats were continuously fed for 90 days.During the experiment,the rats were observed for signs of poisoning (spiritual excitement,loose hair,partial hair loss).Body weight and whole blood were used to measure changes in various blood cell parameters at the end of the treatment time.RESULTS:Compared with the control group,the body weights of the rats exposed to 900 mg/kg DEHP were significantly decreased (P〈0.05).The proportion of toxic signs in the 600 and 900 mg/kg DEHP groups were 63.63% (7/11) and 100% (11/11),which were significantly higher than 0 (0/11) in the control group (P〈0.05).Among DEHP treated rats,the body weights were significantly increased after 1 mg/kg Se intervention (P〈0.05).The signs of poisoning in the 1 mg/kg Se+300 and 600 mg/kg DEHP groups were significantly lower.Compared with the control group,the WBC of the rats exposed to 600 and 900 mg/kg DEHP was significantly lower and the MONO was significantly higher (P〈0.05).Compared with the same dose of DEHP-treated group,the WBC of the 1 mg/kg Se+600 mg/kg DEHP group was significantly increased and the MONO was significantly decreased (P〈0.05).Among the erythrocyte parameters and compared with the control group,the MCH and HDW of the rats were significantly increased after exposure to DEHP;the RBC and HCT of the 600 and 900 mg/kg DEHP treated groups were significantly lower (P〈0.05).Compared with the same dose of DEHP-treated group,there was no significant difference in the red blood cell parameters of the rats after intervention by 1 mg/kg Se.Among the platelet parameters,no DEHP exposed group and Se intervention group had significant effects.CONCLUSION:Exposure of rats to 300-900 mg/kg DEHP affected certain red and white blood cell parameters.Intervention by selenium protected certain blood cell functions under the doses of 300-600 mg/kg DEHP.
作者
杨梅
曹乾
宁俊康
任雪丹
王民生
YANG Mei;CAO Qian;NING Junkang;REN Xuedan;WANG Minsheng(School of Public Health of Southeast University, Nanjing 210009;Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, Jiangsu, China)
出处
《癌变.畸变.突变》
CAS
CSCD
2018年第3期230-233,238,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
江苏省普通高校研究生科研创新计划(SJZZ15_0026)