伴矿景天叶片愈伤组织诱导及植株再生

Callus induction from leaf and plant regeneration of Sedum plumbizincicola

以伴矿景天Sedum plumbizincicola幼嫩叶片为外植体,以添加了300 mg·L^(-1)水解酪蛋白的MS(Murashige and Skoog)培养基为基本培养基,研究不同植物生长调节剂组合对伴矿景天愈伤组织诱导及分化的影响,并建立再生体系。结果表明:1.00 mg·L^(-1)2,4-二氯苯氧乙酸(2,4-D)+0.30 mg·L^(-1) 6-苄基腺嘌呤(6-BA)组合下,愈伤组织诱导效果最佳(P<0.05),诱导率为92.01%;0.10 mg·L^(-1) 2,4-D+1.00 mg·L^(-1) 6-BA组合下,分化效果最优(P<0.05),分化率为27.44%。适宜的芽增殖培养基为0.10 mg·L^(-1) 2,4-D+(0.50~1.00)mg·L^(-1) 6-BA;适宜的生根培养基为1.00~2.00 mg·L^(-1)吲哚丁酸(IBA)。本研究为伴矿景天转基因技术体系的建立奠定了组织培养基础。

To establish the efficient regeneration system and lay foundation for transgenie system of Sedum plumbizincicola in this research, the young leaves of S. plumbizincicola as explants, several factors affecting callus induction, bud differentiation, and plant regeneration were studied, and then a regeneration system was established. An MS medium supplemented with 300 mg·L^-1 hydrolyzed casein was used as the basic medium. Different concentrations of hormone combinations was designed. Results showed that the best medium for leaf callus induction was the basic medium with 1.00 mg·L^-1 2,4-Diehlorophenoxyaeetie acid （2,4-D） and 0.30 mg·L^-1 6-Benzylaminopurine （6-BA） added having a callus induction rate of 92.01%. The best medium for bud differentiation was the basic medium supplemented with 0.10 mg·L^-1 2,4-D and 1.00 mg·L^-1 6-BA having a callus differentiation rate of 27.44%. A suitable medium for bud proliferation was the basic medium with 0.10 mg·L^-1 2,4-D and （0.50-1.00） mg·L^-1 6-BA added. Also, a suitable rooting medium was the basic medium with 1.00-2.00 mg·L^-1 3-indolebutyrie acid （IBA） added. These findings laid a tissue euhure foundation for the establishment of a transgenie system with S. plumbizincicola.