一种转基因马铃薯PCR鉴别方法的建立

Establishment of a PCR method to identify transgenic potato

为建立一种鉴别转基因马铃薯的PCR方法,设计转基因马铃薯常用调控元件的特异性引物,以4种转基因马铃薯标准品系EH92-527-1、AV43-6-G7、AM04-1020、PH05-026-0048为模板进行单基因PCR和多重PCR扩增工艺方法研究。经琼脂糖凝胶电泳结果分析后,从中筛选出一组引物特异性较强,且无交叉污染或非特异性扩增现象的引物组合对。对9种已知转基因马铃薯样品进行多重PCR验证,均得到条带清晰且区分明显的目的条带。成功建立了能够快速、准确鉴别转基因马铃薯中多种调控元件的多重PCR与单基因PCR相结合的检测方法。

A PCR method to identify transgenic potato was established by designing specific primers of regulatory elements in genetically modified organism（ GMO） potato,then four kinds of transgenic potato standard lines EH92-527-1,AV43-6-G7,AM04-1020 and PH05-026-0048 were used as templates for single gene PCR and multiplex PCR amplification. After analysis by agarose gel electrophoresis,the primer combinations with strong primer specificity and no cross-contamination or non-specific amplification were screened out. And nine kinds of known transgenic potato samples were verified by multiplex PCR,and the bands were clear and distinct. The detection method of combining multiplex PCR with single PCR that can rapidly and accurately identify a variety of regulatory elements of transgenic potatoes has been established.