Nogo基因rs2920880和rs17046583多态性研究

Study on rs2920880 and rs17046583 polymorphisms of Nogo gene

目的研究Nogo基因rs2920880、rs17046583位点在广西人群中分布特点,并与不同人群比较。方法经多重单碱基延伸法、DNA测序法分析323例广西人群rs2920880和rs17046583的基因型,分析基因型及等位基因频率在性别及不同人群中的分布差异。结果 rs2920880存在CC、CT、TT三种基因型,频率为1.9%、17.0%、81.1%。rs17046583存在AA、AG、GG三种基因型,频率为65.6%、29.1%、5.3%。广西人群两位点的基因型、等位基因在男女之间分布差异无统计学意义(P>0.05)。而与欧洲人、非洲人和日本人群相比,分布差异均有统计学意义(P<0.01)。结论 Nogo基因rs2920880和rs17046583多态性在不同人群间存在着不同程度差异,这种差异在疾病的关联性研究中可能具有重要指导意义。

Objective To study the distribution characteristic of rs2920880 and rs17046583 loci of Nogo gene in Guan- gxi,and compare with different populations.Methods The genotypes of rs2920880 and rs17046583 were analyzed by using multiple single base extension method and DNA sequencing in 323 Guangxi people.And the distribution differences of genotypes and genotype frequencies in gender and different populations were analyzed. Results CC, CT, and TT were found in rs2920880 with the allele frequency of 1.9%, 17.0% and 81.1% ,respectively.AA,AG and GG were found in rs17046583 with the genotype frequency of 65.6%, 29.1%, and 5.3 %, respectively.There was no statistically significant difference in genotypes and genotype between men and women at the two above-mentioned loci in Guangxi（ P〉0.05 ） .While there was statistically significant difference of genotypes and alleles among Guangxi population, HapMap-CEU, HapMap-YRI and HapMap-JPT （P〈0.01）.Conclusion rs2920880 and rs17046583 polymorphism of Nogo gene are varied in different populations, which may have great important guiding significance in relevance research on diseases.