脑络欣通对血管性痴呆大鼠学习记忆功能及海马神经元内钙离子浓度的影响

Effects of Naoluo Xintong Decoction on vascular dementia rats' memory and learning,and hippocampal neuronal intracellular calcium concentration

目的观察脑络欣通对血管性痴呆(VD)模型大鼠学习记忆功能的作用及对大鼠海马神经元胞内钙离子水平的影响。方法采取改良双侧颈总动脉结扎方法 (2-VO)造模血管性痴呆大鼠;随机分为模型组、脑络欣通组(8.54 g/kg)、石杉碱甲组(0.06 mg/kg)及假手术组;大鼠灌胃给药1月后,Morris水迷宫实验检测大鼠学习记忆能力;流式细胞仪检测海马神经元胞内钙离子荧光强度;酶联免疫吸附试验(ELISA)检测大鼠海马组织降钙素基因相关肽及其受体的表达。结果与模型组比较,脑络欣通可明显缩短血管性痴呆模型大鼠的逃避潜伏期(P<0.01);可显著增加血管性痴呆模型大鼠停留在站台所在象限时间及穿越平台的次数(P<0.01);与模型组比较,脑络欣通可显著减低血管性痴呆模型大鼠海马神经元胞内Ca^(2+)水平(P<0.01);与模型组比较,脑络欣通可增强血管性痴呆模型大鼠海马组织降钙素基因相关肽及其受体的表达(P<0.05)。结论脑络欣通可显著改善血管性痴呆模型大鼠学习记忆能力,可能与通过增强大鼠海马组织降钙素基因相关肽及其受体表达进而降低海马神经元内Ca^(2+)水平有关。

AIM To study the effects of Naoluo Xintong Decoction（ NLXTD） on vascular dementia（ VD）rats＇ memory and learning,and hippocampal neuronal intracellular calcium concentration. METHODS Rats were divided randomly into model group,NLXTD group（8. 54 g/kg）,Huperzia-A group（0. 06 mg/kg） and sham group. They were made into vascular dementia rats by the improved bilateral carotid artery ligation method（2-VO）thereafter if necessary. After one-month corresponding intragastric administration,the rats were ethologically evaluated by the Morris water maze experiment; their fluorescence intensity of hippocampal neuronal intracellular calcium concentration was determined by flow cytometry,and the expression levels of calcitonin gene related peptide（ CGRP） and its hippocampus receptor were detected by enzyme-linked immunosorbent assay（ ELISA）.RESULTS Compared with the model group,rats in NLXTD group displayed overall superiority to those of the model group in terms of significantly shortened time of escape latency（ P〈0. 01）,significantly increased number through the platform and the times in the fourth quadrant（ P〈0. 01）,a lower fluorescence intensity indicating a lower hippocampal neuronal intracellular calcium concentration（ P〈0. 05）. CONCLUSION The significant improvement of memory and learning observed among VD rats＇ due to NLXTD intervention may be attributable to its efficacy in reducing the hippocampal neuronal intracellular calcium concentration by enhancing the expression levels of CGRP and its receptor.