葛根芩连丸UPLC指纹图谱建立及7种成分测定

Establishment of UPLC fingerprints of Gegen Qinlian Pills and determination of seven constituents

目的建立葛根芩连丸(葛根、黄芩、黄连、炙甘草)UPLC指纹图谱,并测定葛根素、黄芩素、巴马汀、汉黄芩素、大豆苷、大豆苷元、药根碱的含有量。方法该药物70%甲醇提取液的分析采用Waters ACQUITY UPLC~ BEH C_(18)色谱柱(2.1 mm×50 mm,1.7μm);甲醇-0.1%冰醋酸为流动相,梯度洗脱;体积流量0.3 m L/min;检测波长260 nm;柱温30℃。结果 10批样品指纹图谱中有25个共有峰,相似度大于0.98。7种成分在各自范围内线性关系良好(r>0.999 0),平均加样回收率98.99%~103.6%,RSD 1.13%~2.03%。结论该方法简便可靠,重复性好,可用于葛根芩连丸的质量控制。

AIM To establish the UPLC fingerprints of Gegen Qinlian Pills（ Puerariae lobatae Radix,Scutellariae Radix,Coptidis Rhizoma and Glycyrrhizae Radix et Rhizoma Praeparata cum Melle） and to determine the contents of puerarin,baicalein,palmatine,wogonin,daidzin,daidzein and jateorhizine. METHODS The analysis of 70% methanol extract of this drug was performed on a 30 ℃ thermostatic Waters ACQUITY UPLC BEH C18 column,with the mobile phase comprising of methanol-0. 1% glacial acetic acid flowing at 0. 3 m L/min in a gradient manner,and the detection wavelength was set at 260 nm. RESULTS There were twenty-five common peaks in the fingerprints of ten batches of samples with the similarities of more than 0. 98. Seven constituents showed good linear relationships within their own ranges（ r〉0. 999 0）,whose average recoveries were 98. 99%-103. 6% with the RSDs of 1. 13%-2. 03%. CONCLUSION This simple,reliable and reproducible method can be used for the quality control of Gegen Qinlian Pills.