银柴胡化学成分及其抗炎活性

Chemical constituents from Stellaria dichotoma and their anti-inflammatory activities

目的研究银柴胡Stellaria dichotoma L.var.lanceolata Bge的化学成分及其抗炎活性。方法银柴胡95%乙醇提取物采用硅胶、MCI、反相C18制备液相色谱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用脂多糖(LPS)诱导的小鼠巨噬细胞RAW264.7炎症反应模型评价其抗炎活性。结果从中分离得到10个化合物,分别鉴定为3-羟基-β-卡巴林(1)、taraxacine A(2)、1,2,3,4-四氢-1,3,4-三羟基-β-卡巴林(3)、1-乙酰基-β-卡巴林(4)、arenarine A(5)、arenarine B(6)、邻苯二甲酸二异丁酯(7)、邻苯二甲酸二丁酯(8)、亚麻酸甲酯(9)、苜蓿素(10)。化合物1~6对炎症因子有较强的抑制作用,IC504.79~9.34μg/m L。结论所有化合物均为首次从该植物中分离得到,首次发现化合物1~6有较强的抗炎活性。

AIM To study the chemical constituents from the Stellaria dichotoma L. var. lanceolata Bge and their anti-inflammatory activities. METHODS The 95% ethnol extract from S. dichotoma was isolated and purified by silica,MCI,C18 prep-HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data. Their anti-inflammatory activities were evaluated by LPS induced RAW264. 7 inflammatory cell model. RESULTS Ten compounds were isolated and identified as 3-hydroxy-β-carboline（1）,taraxacine A（2）,1,2,3,4-tetrahydro-1,3,4-trioxo-β-carbo1 ine（ 3）,1-acetyl-β-carboline（ 4）,arenarine A（5）,arenarine B（6）,diisobutyl-phthalate（7）,dibutyl-phthalate（8）,（z,z,z）-9,12,15-octadecatrienoic acid,methyl ester（9）,tricin（10）. Compounds 1-6 had stronger anti-inflammatory activities with the IC50 values of 4. 79-9. 34 μg/m L. CONCLUSION All the compounds are isolated from this plant for the first time.Their stronger anti-inflammatory activities are discovered for the first time.