期刊文献+

GC法同时测定不同种类苍术中6种成分 被引量:13

Simultaneous determination of six constituents in different kinds of Atractylodes rhizome by GC
下载PDF
导出
摘要 目的建立GC法同时测定不同种类苍术Atractylodes rhizome中β-桉叶醇、苍术酮、苍术素、白术内酯Ⅰ、白术内酯Ⅱ和(4E,6E,12E)-十四癸三烯-8,10-二炔-1,3-二乙酸酯的含有量,根据含有量水平对苍术药材进行聚类分析。方法苍术溶液的分析采用HP-5毛细管柱(30 m×0.32 mm,0.25μm);起始温度100℃,以10℃/min升温至135℃,再以1℃/min升温至150℃;再以5℃/min升温至200℃,保持6 min;再以50℃/min升温至250℃,保持8 min。FID检测器温度300℃;进样口温度250℃;载气体积流量1.4 L/min;尾吹气N_2(99.999%),载气比例Air∶H_2∶N_2=400∶30∶25;进样量1μL;分流比20∶1。采用SPSS 21.0统计软件进行聚类分析。结果 6种成分分别在各自范围内线性关系良好(r﹥0.999 6),平均回收率99.46%~100.95%,RSD 0.09~0.41,所分析的苍术分为3类。结论该方法准确稳定,重复性好,可用于苍术的质量控制。 AIM To establish a GC method for the simultaneous determination of β-eudesmol,atractylon,atractylodin,atractylolide Ⅰ,atractylaxanthin II and(4 E,6 E,12 E)-tetradecene-8,10-diyne-1,3-diacetate in Atractylodes rhizome,and cluster analysis of A. rhizome according to the content level. METHODS The analysis of A. rhizome solution was performed on an HP-5 capillary column(30 m × 0. 32 mm,0. 25 μm) with FID as the detector,the initial temperature 100 ℃,with 10 ℃/min to 135 ℃; with 1 ℃/min to 150 ℃; with 50 ℃/min to200 ℃( keeping 6 minutes),and with 50 ℃/min to 250 ℃( keeping 8 minutes). The FID detector temperature was 300 ℃ and the injector temperature was 250 ℃,with the flow rate carrier gas 1. 4 L/min; The tail gas was N_2(99. 999%),with the ratio of carrier gas Air ∶ H_2∶ N_2= 400 ∶ 30 ∶ 25; The sample volume was 1 μL,and the split ratio was 20 ∶ 1. The results were analyzed by cluster analysis with SPSS 21. 0 statistical software.RESULTS Six constituents showed good linear relationships within their own ranges( r〉0. 999 6),whose average recoveries were 99. 46%-100. 95% with the RSDs of 0. 09-0. 41. A. rhizome was divided into three categories. CONCLUSION This accurate, stable and reproducible method can be used for the quality control of A. rhizome.
作者 陈文婷 陈阳 张意涵 李琴瑜 范燕豪 安叡 侯剑伟 王新宏 CHEN Wen-ting;CHEN Yang;ZHANG Yi-han;LI Qin-yu;FAN Yan-hao;AN Rui;HOU Jian-wei;WANG Xin-hong(Shanghai University of Traditional Chinese Medicine,Shanghai 201203, China)
机构地区 上海中医药大学
出处 《中成药》 CAS CSCD 北大核心 2018年第5期1124-1129,共6页 Chinese Traditional Patent Medicine
基金 国家中药标准化项目(ZYBZH-Y-HEB-15)
关键词 苍术 化学成分 聚类分析 Atractylodes rhizome chemical constituents cluster analysis
  • 相关文献

参考文献13

二级参考文献128

共引文献242

同被引文献151

引证文献13

二级引证文献63

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部