摘要
目的建立GC法同时测定不同种类苍术Atractylodes rhizome中β-桉叶醇、苍术酮、苍术素、白术内酯Ⅰ、白术内酯Ⅱ和(4E,6E,12E)-十四癸三烯-8,10-二炔-1,3-二乙酸酯的含有量,根据含有量水平对苍术药材进行聚类分析。方法苍术溶液的分析采用HP-5毛细管柱(30 m×0.32 mm,0.25μm);起始温度100℃,以10℃/min升温至135℃,再以1℃/min升温至150℃;再以5℃/min升温至200℃,保持6 min;再以50℃/min升温至250℃,保持8 min。FID检测器温度300℃;进样口温度250℃;载气体积流量1.4 L/min;尾吹气N_2(99.999%),载气比例Air∶H_2∶N_2=400∶30∶25;进样量1μL;分流比20∶1。采用SPSS 21.0统计软件进行聚类分析。结果 6种成分分别在各自范围内线性关系良好(r﹥0.999 6),平均回收率99.46%~100.95%,RSD 0.09~0.41,所分析的苍术分为3类。结论该方法准确稳定,重复性好,可用于苍术的质量控制。
AIM To establish a GC method for the simultaneous determination of β-eudesmol,atractylon,atractylodin,atractylolide Ⅰ,atractylaxanthin II and(4 E,6 E,12 E)-tetradecene-8,10-diyne-1,3-diacetate in Atractylodes rhizome,and cluster analysis of A. rhizome according to the content level. METHODS The analysis of A. rhizome solution was performed on an HP-5 capillary column(30 m × 0. 32 mm,0. 25 μm) with FID as the detector,the initial temperature 100 ℃,with 10 ℃/min to 135 ℃; with 1 ℃/min to 150 ℃; with 50 ℃/min to200 ℃( keeping 6 minutes),and with 50 ℃/min to 250 ℃( keeping 8 minutes). The FID detector temperature was 300 ℃ and the injector temperature was 250 ℃,with the flow rate carrier gas 1. 4 L/min; The tail gas was N_2(99. 999%),with the ratio of carrier gas Air ∶ H_2∶ N_2= 400 ∶ 30 ∶ 25; The sample volume was 1 μL,and the split ratio was 20 ∶ 1. The results were analyzed by cluster analysis with SPSS 21. 0 statistical software.RESULTS Six constituents showed good linear relationships within their own ranges( r〉0. 999 6),whose average recoveries were 99. 46%-100. 95% with the RSDs of 0. 09-0. 41. A. rhizome was divided into three categories. CONCLUSION This accurate, stable and reproducible method can be used for the quality control of A. rhizome.
作者
陈文婷
陈阳
张意涵
李琴瑜
范燕豪
安叡
侯剑伟
王新宏
CHEN Wen-ting;CHEN Yang;ZHANG Yi-han;LI Qin-yu;FAN Yan-hao;AN Rui;HOU Jian-wei;WANG Xin-hong(Shanghai University of Traditional Chinese Medicine,Shanghai 201203, China)
出处
《中成药》
CAS
CSCD
北大核心
2018年第5期1124-1129,共6页
Chinese Traditional Patent Medicine
基金
国家中药标准化项目(ZYBZH-Y-HEB-15)
关键词
苍术
化学成分
聚类分析
Atractylodes rhizome
chemical constituents
cluster analysis