瓶式平板培养制备稻曲病菌薄壁分生孢子

Culture method with plate in flask for sporulation of thin-walled conidia of Villosiclava virens

利用三角瓶装纳马铃薯琼脂培养基制备稻曲病菌薄壁分生孢子。结果发现,瓶装培养基的体积与产孢量无关。各菌株在瓶式培养平板上均能正常形成后代孢子。瓶式培养比常规培养皿平板培养具有更高的产孢效率。产孢平板上产生的孢子收取后,菌落继续培养,新形成的孢子数量大幅下降。由于三角瓶能高效避免污染,因而认为瓶式培养是制备稻曲病菌薄壁分生孢子的理想技术。

A culture method with potato agar plate in flask（flask method）for sporulation of thin-walled conidia of Villosiclava virens was established.The results indicated that no relations existed between conidial yield and medium volume of the potato agar inside the flask.Conidia of new generation were formed normally by the flask method for all isolates examined.Efficiency of sporulation cultured by plate in the flask was higher than that by plate inside a common dish.Conidial yield of new generation was decreased greatly when a plate colony was cultured repeatedly after the conidia were moved out.It was concluded that the flask method was an ideal technique for sporulation culture of thin-walled conidia of V.virens due to the high efficiency of sporulation as well as anticontamination.