硒蛋白S基因单核苷酸多态性与肝癌发病风险的关联性分析

Analysis on association between single nucleotide polymorphism of selenoprotein S gene and risk of liver cancer

目的:探讨内蒙古自治区汉族人群硒蛋白S(SelS)基因单核苷酸多态性(SNP)在肝癌患者和健康对照者的分布情况,阐明其与肝癌发病风险的关联性。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测内蒙古自治区汉族肝癌患者(n=96)和健康对照者(n=135)SelS基因rs34713741位点基因型和等位基因频数分布,结果经测序验证。分析SelS基因SNP与肝癌发病风险的关系。结果:SelS基因rs34713741位点的3种基因型CC、CT、TT及等位基因C、T的频数分布在肝癌组和健康对照组间比较差异均无统计学意义(χ~2=2.679,χ~2=2.331,P>0.05)。肝癌组和健康对照组饮酒者CC、CT、TT基因型和等位基因C、T频数分布比较差异均有统计学意义(χ~2=6.533,χ~2=6.417,P<0.05);携带T等位基因的饮酒个体患肝癌的风险增加,是CC基因型的2.297倍(OR=2.297,95%CI:1.202~4.393)。结论:SelS基因和饮酒交互作用可能与内蒙古汉族人群肝癌的发病有关联。

Objective：To investigate the distribution of single nucleotide polymorphism（SNP）of selenoprotein S（SelS）gene in the patients with liver cancer and the healthy controls,and to clarify the correlation of the SNP and the risk of liver cancer in the Inner Mongolia Han population.Methods：The distribution of genotypic and allelic frequencies at rs34713741 site was detected by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） method among 96 patients with liver cancer and 135 healthy controls,and verified by gene sequencing analysis.The relationship between SNP of SelS gene and the risk of liver cancer was analyzed.Results：There were no differences of the frequencies of CC,CT,and TT genotypes and C and T alleles at rs34713741 site of SelS gene between the patients with liver cancer and the healthy controls（χ-2=2.679,χ-2=2.331,P〈0.05）.The differences in the frequencies of CC,CT,and TT genotypes and C and T alleles at rs34713741 site of SelS gene of the drinkers between liver cancer group and healthy control group were significant（χ-2=6.533,χ-2=6.417,P〈0.05）.The relative risk of liver cancer in the drinkers carrying T allele was 2.297 times of the drinkers carrying genotype CC（OR = 2.297, 95% CI： 1.202-4.393）.Conclusion：The gene-environment interaction of SelS and drinking may associate with liver cancer in the Inner Mongolia Han population.