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荧光法快速测定乳品中恩诺沙星的含量

Rapid Determination of Enrofloxacin in Dairy Products by Fluorescence Spectrometry
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摘要 以乙腈为提取溶剂,选择超声波提取方法,建立起荧光法快速测定乳品样品中恩诺沙星含量的新方法。采用标准曲线法测定,当恩诺沙星浓度在1~3000ng·mL^(-1)范围内,线性方程为F=2.60C+116.37,相关系数R=0.9993,方法的检出限为0.18 ng·mL^(-1),回收率为89.66%~101.18%。该测定方法简单、快速、准确、灵敏,用于实际乳品中恩诺沙星含量的测定,结果满意。 The acetonitrile is used as the extraction solvent and the ultrasonic extraction method is selected in the measurement of the dairy products. A new method of fluorescence spectrometry to rapid determine enrofloxacin in dairy products is developed. The standard curve method is adopted. When enrofloxacin concentration in the range of 1~3 000 ng·mL^-1, the linear equation is F = 2.60 C +116.37, the correlation coefficient is 0.9993 and the detection limit of 0.18 ng·mL^-1. The recovery is in the range of 89.66 % ~101.18 %. This method is simple, rapid, high accuracy and sensitive, which has been successfully applied to determine the enrofloxacin in dairy products with satisfactory results.
作者 蔡其洪 陈小萍 赵成飞 沈毅萍 侯慧青 王洁婷 CAI Qihong;CHEN Xiaoping;ZHAO Chengfei;SHEN Yiping;HOU Huiqing;WANG Jieting(School of Pharmacy and Medical Technology, Putian University, Putian Fujian 351100, China)
出处 《莆田学院学报》 2018年第2期57-60,共4页 Journal of putian University
基金 福建省科技计划引导性项目(2015Y0030) 福建省教育厅科技项目(JK2014045) 莆田市科技计划项目(2013S01(2))
关键词 荧光分析法 乳品 恩诺沙星 fluorescence spectrometry dairy products enrofloxacin
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