反相高效液相色谱法测定人血清中奥卡西平活性代谢产物的浓度

Determination of active metabolites of oxcarbazepine in human serum by reversed-phase high performance liquid chromatography

目的建立反相高效液相色谱法测定人血清中奥卡西平(OXC)活性代谢产物MHD的浓度。方法收集血清样品经甲醇沉淀蛋白后,采用Inert Sustain C18(5μm,4.6×150 mm)色谱柱,流动相为甲醇-水(35:65),流速:0.8 mL/min,检测波长:210 nm,柱温:40℃,进样体积:20μL。结果血清中内源性物质对样品测定无干扰,MHD在4.8~38.4μg/mL线性关系良好,回归方程为:A=13968.3C-13730.7,r=0.9990。MHD的低、中、高3种浓度平均回收率均>95%,日内、间RSD均<3%(n=5)。结论反相高效液相色谱法简便、准确、灵敏度高,适用于人体内奥卡西平活性代谢产物MHD的血药浓度监测。

Objective To establish reversed-phase high performance liquid chromatography method for the determina- tion of oxcarbazepine（OXC） active metabolite MHD concentration in human serum. Methods The serum samples were collected and the protein was precipitated by methanol, then InertSustain C 18 （5 μm, 4.6×150 mm） chromatography column was used, and the mobile phase was methanol-water（35：65）, flow rate： 0.SmL/min, detection wavelength： 210 nm, column temperature： 40℃, injection volume： 20 μL. Results The endogenous substances in serum did not interfere with the determination of the samples. The linearity of MHD was good from 4.8 to 38.4 μg/mL. The regression equation was： A =13968.3C-13730.7, r=0.9990. The average recoveries of MHD at low, medium, and high concentrations were all〉 95%, and intra-and inter-day RSDs were 〈3%（n=5）. Conclusion The reversed-phase high performance liquid chromatography method is simple, accurate and sensitive, which is suitable for monitoring the plasma concentration of MHD, an active metabolite of oxcarbazepine.