摘要
从位于中国东海的钢铁研究总院舟山海洋腐蚀研究所近海海域的海底沉积物中分离提取得到杀鱼假交替单胞菌,分别将其接种到细菌培养广口瓶和模拟海水流动环境培养器中,利用电化学工作站、扫描电镜及红外光谱仪对浸泡于两种培养体系中Q235碳钢样品的腐蚀行为进行研究。结果表明,杀鱼假交替单胞菌能抑制碳钢在海水中的腐蚀进程。样品的阻抗在广口瓶培养体系中得到了更为明显的提高,广口瓶培养体系中的碳钢样品表面覆盖着一层质地均匀且完整的生物膜;而模拟海水流动环境中碳钢样品表面的生物膜厚度不均匀,海水通过膜层上的孔洞和裂隙与未成膜基体直接接触,在生物膜与样品表面之间形成氧浓差微电池使得局部腐蚀得以发展。红外光谱分析结果显示,培养7 d后两个体系中碳钢样品表面的生物膜内细菌的分泌物大分子不同。
Pseudoalteromonas piscicida was separated and extracted from seafloor sediments locat- ed in offshore waters of Zhoushan institute of marine corrosion at Zhoushan islands of the East China Sea. Then the bacterium was inculated to laboratorial bottle that placed in a constant temperature incuba- tor shaker and to an incubator with environment of simulated lowing sea water, respectively. The effect of Pseudoalteromonas piscicida on the corrosion of Q235 carbon steel in the above two bacterial culture systems has been studied by means of electrochemical workstation, scanning electron microscope andFourier infrared spectrometer. Results showed that this bacterium could inhibit effectively the corrosion of Q235 carbon steel in seawater. The impedance of the carbon steel was enhanced more obviously in labo- ratorial bottle and its surface was completely covered with an uniform and dense biofllm, while the un- even biofllm formed on the surface of carbon steel in the simulated flowing seawater system, seawater can direct contact the substrate via holes and crevices which were randomly distributed throughout the biofilm, provided conditions for the formation of oxygen concentration cell, therefore weakened the corro- sion resistance of carbon steel. FT-IR spectrum showed there were differences in secretory macromole- cules for the same bacterium but cultured respectively in the two culture systems after 7 d.
作者
叶赛
Masoumeh Moradi
宋振纶
胡方勤
孙朝晖
龙剑平
YE Sai;Masoumeh Moradi;SONG Zhenlun;HU Fangqin;SHUN Zhaohui;LONG Jianping(College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu 610059, China;Key Laboratory of Marine New Materials and Related Technology, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China)
出处
《中国腐蚀与防护学报》
CAS
CSCD
北大核心
2018年第2期174-182,共9页
Journal of Chinese Society For Corrosion and Protection
基金
国家自然科学基金(5161101078)
中国科学院院长国际奖学金(PIFI)
浙江省公益项目(2015C31031)
宁波市自然科学基金(2015A610070)~~
关键词
杀鱼假交替单胞菌
抑制腐蚀
实验室条件培养
模拟海洋流动环境培养
氧浓差电池
Pseudoalteromonas piscicida
corrosion inhibition
laboratorial culturing
simulatedmarine environmental culturing
oxygen concentration cell