迁移侵袭抑制蛋白和p21活化激酶1在子宫内膜癌中的表达及其临床意义

Expression and clinical significance of MIIP and PAK1 in endometrial carcinoma

目的探讨迁移侵袭抑制蛋白（MIIP）和p21活化激酶1（PAK1）在子宫内膜癌组织中的表达，分析其与子宫内膜癌患者临床病理特征及预后的关系。方法采用免疫组化SP法检测135例子宫内膜癌、55例不典型增生和88例正常子宫内膜组织中MIIP和PAK1蛋白的表达，分析子宫内膜癌组织中MIIP和PAK1蛋白表达的临床意义及二者的相关性。 结果正常子宫内膜组、不典型增生组和子宫内膜癌组MIIP的阳性表达率分别为52.3%（46/88）、41.8%（23/55）和34.8%（47/135），子宫内膜癌组明显低于正常子宫内膜组（P〈0.05）。MIIP蛋白的表达与肌层浸润、国际妇产科联盟（FIGO）分期和淋巴结转移情况有关（均P〈0.05）。正常子宫内膜组、不典型增生组和子宫内膜癌组的PAK1阳性表达率分别为45.5%（40/88）、50.9%（28/55）和62.2%（84/135），子宫内膜癌组显著高于正常子宫内膜组（P〈0.05）。PAK1蛋白的表达与组织学分级、肌层浸润、FIGO分期和淋巴结转移情况有关（均P〈0.05）。MIIP和PAK1蛋白的表达与患者的累积生存率无关（P值分别为0.092和0.052）。子宫内膜癌组织中MIIP与PAK1蛋白的表达呈负相关（r=-0.329，P〈0.001）。结论MIIP的低表达和PAK1的过表达共同参与了子宫内膜癌的发生、发展，与子宫内膜癌的不良预后有关。MIIP和PAK1蛋白在子宫内膜癌中的表达呈负相关。

ObjectiveTo investigate the expressions of migration and invasion inhibitory protein （MIIP） and p21-activated kinase 1 （PAK1） in endometrial carcinoma （EC） and their correlation with clinicopathological features.Methods The protein levels of MIIP and PAK1 in 135 paraffin-embedded EC tissues, 55 atypical hyperplasia of endometrium （AHE） and 88 normal endometrium （NE） tissues were quantified by immunohistochemistry, the clincial significance and the relationship of these two proteins were also analyzed.ResultsThe positive rates of MIIP expression in NE, AHE and EC tissues were 52.3%（46/88）, 41.8% （23/55） and 34.8% （47/135）, respectively. The expression of MIIP in EC was significantly lower than that of MIIP in NE （P〈0.05）. The positive rates of PAK1 expression in NE, AHE and EC tissues were 45.5% （40/88）, 50.9% （28/55） and 62.2% （84/135）, respectively. The expression of PAK1 in EC tissues was significantly higher than that of PAK1 in NE tissues （P〈0.05）. The expression of MIIP in EC tissues was significantly associated with myometrial invasion, International Federation of Gynaecology and Obstetrics （FIGO） stage and lymph node metastasis （P〈0.05）. The expression of PAK1 in EC tissues was significantly related with differentiation, myometrial invasion, FIGO stage and lymph node metastasis （P〈0.05）. The expressions of MIIP and PAK1 in EC tissues were marginally related with the overall survival of patients （P=0.092, P=0.052）. The expression of MIIP in EC was negatively correlated with PAK1 （r=-0.329, P〈0.001）.Conclusions The down-regulation of MIIP and up-regualtion of PAK1 paticipate in the initiation and development of EC, which are correlated with the poor prognosis of EC. The protein expression of MIIP is inversely related with PAK1 in EC.