海绵共生真菌产黄青霉LS16抗菌活性物质的分离及结构鉴定

Isolation and structure elucidation of antibacterial metabolites from a sponge-derived fungus Penicillium chrysogenum LS16

海洋真菌能够产生大量活性独特的次级代谢产物。为了探明海绵共生真菌产黄青霉LS16发酵液中抗副溶血弧菌Vibrio parahemolyticus的活性物质,本实验对副溶血弧菌Vibrio parahemolyticus的抑菌活性进行跟踪,采用VLC(vacuum liquid chromatography)、Sephadex LH-20柱层析、薄层层析和高效液相色谱等技术,从海绵共生真菌LS16乙酸乙酯发酵液中分离纯化得到5个化合物。进一步实验证明,化合物2具有抗副溶血弧菌Vibrio parahemolyticus活性。根据该化合物的波谱数据(1H NMR、13C NMR)对其化学结构进行鉴定,确定其分子式为C15H15NO3,为生物碱类化合物。

Marine fungi are prolific in production of unique secondary metabolites with promising biological activities. In an effort to find the compounds with anti-Vibrio parahemolyticus activity from the fermentation broth of a marine fungal strain Penicillium chrysogenum LS16, the ethyl acetate extract were fractionated sequentially by silica gel VLC, Sephadex LH-20 column chromatography, and reversed-phase HPLC combined with bioassay-directed isolation. Five compounds were afforded, among them compound 2 displayed potent inhibition against Vibrio parahemolyticus. The structure of compound 2 was elucidated as an alkaloid on the basis of spectroscopic analysis（^1 H NMR and ^13C NMR）, which is in agreement with the molecular formula C15H15NO3.