GNM C7-8实时荧光定量PCR同时快速检测8种食源性致病菌

Simultaneous rapid detection of 8 kinds of foodborne bacteria by GNM C7-8 real-time PCR

目的基于GNM C7-8实时荧光定量PCR系统实现对8种常见食源性病原菌的同时快速检测。方法以建立的8种常见食源性致病菌的基于内参系统的实时荧光PCR检测方法为参照,基于GNM C7-8实时荧光定量PCR系统,对上述病原菌进行同一时间点和不同时间点的同时快速检测,并将检测结果同常见的ABI7500和罗氏荧光定量PCR仪检测结果进行比较。结果基于八模块设计的GNM C7-8实时荧光定量PCR系统能够在同一时间点和不同时间点启动运行,并实现对8种病原菌的同时快速检测;不同病原体的检测体系独立运行,互不干扰,不易发生交叉污染;与其他2种荧光定量PCR仪相比,扩增结果一致,但是完成检测所需时间更短。结论对于食物中毒和疫病爆发等突发性重大公共卫生事件的应急处置,GNM C7-8实时荧光定量PCR系统将可以提供强有力的设备支持。

Objective To establish a method for simultaneous rapid detection of 8 kinds of foodborne bacteria by GNM C7-8 real-time PCR system. Methods With the real-time PCR assays based on the internal amplification control （IAC） for 8 kinds of foodborne bacteria as the reference, which had been developed, the simultaneous detections were performed at the same time or at a certain interval through the GNM C7-8. Then the detection results including the detection time were compared to ABI 7500 and Roche real-time PCR machines. Results GNM C7-8 real-time PCR system with 8 modules worked well when it was running either at the same time or at a certain interval for simultaneous detection the 8 kinds of bacteria. The different reaction systems for different bacteria were running independently in different modules, and did not interfere with each other. The design greatly reduced the probability of cross contamination during the detection process. When compared to the other 2 PCR machines, GNM C7-8 demonstrated the similar amplification results while required much less amplification time. Coneluslons GNM C7-8 real-time PCR system can be used in the emergency treatments for the unexpected major public health events, such as food poison and disease outbreaks, which will provide powerful supports in respect of detecting equipments.