SAT技术在沙眼衣原体和解脲脲原体检测中的应用

Application of SAT technique in detection of Chlamydia trachomatis and Ureaplasma urealyticum

目的 了解广州地区沙眼衣原体（CT）和解脲脲原体（UU）的感染现状,同时分析实时荧光核酸恒温扩增检测技术（SAT）与常规方法（乳胶免疫层析法检测CT和液体培养法检测UU）结果的一致性.方法 收集2015年1月至2016年12月来中山大学孙逸仙纪念医院就诊患者的泌尿生殖道分泌物或尿液标本共12120份,采用SAT技术对以上标本进行CT-RNA、UU-RNA检测,同时收集其中部分患者标本进行常规方法检测.采用χ2检验比较SAT技术与常规方法检测CT和UU的结果分别在不同性别和不同年龄段间的阳性率,同时采用Kappa检验分析不同检测方法结果的一致性.结果 2015至2016年广州地区的CT 阳性率为4.05%（356/8781）,UU阳性率为33.69%（1125/3339）,UU阳性率显著高于CT,差异具有统计学意义（χ^2=1981,P〈0.01）.145份CT检测标本中,SAT法和乳胶免疫层析法结果的符合率为96.55%（140/145）,一致性强度为高度（Kappa值为0.65）.186份UU检测标本中,SAT法和液体培养法结果的符合率为92.47%（172/186）,一致性极强（Kappa值为0.81）.结论 广州地区UU阳性率显著高于CT,应用SAT法与常规方法检测CT和UU的结果一致性较高,能为临床诊断CT和UU感染提供依据.

Objective To study the Chlamydia trachomatis（CT）and Ureaplasma urealyticum （UU）infection in Guangzhou area, and analyze the consistency of simultaneous amplification and testing （SAT）and conventional methods（CT was detected by latex immunochromatography, UU was detected by liquid culture method）.Methods A total of 12 120 samples of urogenital secretions or urine samples were collected from Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University from January 2015 to December 2016.CT-RNA and UU-RNA were detected by the SAT technique, a part of samples were tested by conventional methods at the same time.The positive rates of CT and UU by SAT and the conventional methods between different gender and age groups were analyzed by χ^2test, the consistencies between different detection methods were analyzed by Kappa test.Results The positive rate of CT was 4.05%（356/8 781）, UU 33.69%（1 125/3 339）in Guangzhou from 2015 to 2016.The positive rate of UU was significantly higher than that of CT（χ^2=1 981,P〈0.01）.Of 145 specimens for CT test,the coincidence rate between SAT and latex immunochromatographic method was 96.55%（140/145）, which showed good consistency（Kappa=0.65）.Of 186 specimens for UU test,the coincidence rate of the results between the SAT method and liquid culture was 92.47%（172/186）,which showed strong consistency（Kappa=0.81）. Conclusions The positive rate of UU was significantly higher than that of CT in Guangzhou.The SAT method and conventional methods to detect CT and UU show high consistency, which can provide the evidence for clinical diagnosis of CT and UU infection.