雄激素对小鼠睾丸支持细胞Akt和MEK/Erk磷酸化水平的影响

The Effect of Androgens on Akt and MEK/Erk Phosphorylation in Mouse Testicular Sertoli Cells

磷酸化信号通路在精子发生过程中有重要作用,本文研究小鼠睾丸支持细胞中雄激素诱导的蛋白磷酸化。研究中分离培养小鼠睾丸支持细胞,建立了稳定的培养体系。蛋白磷酸化抗体芯片结果显示,睾酮快速激活Akt及MEK/Erk的磷酸化,蛋白免疫印迹验证了芯片分析结果,且激酶磷酸化的激活是由AR介导。结果表明睾酮诱导小鼠睾丸支持细胞中Akt和MEK/Erk磷酸化。

Phosphorylation signaling pathway is critical for spermatogenesis, and the paper studied the proteinphosphorylation induced by androgen in mouse Sertoli cells. Sertoli cells were isolated from mouse testes,and we built astable culture system for the primary cultured cells. Protein phosphorylation signaling pathway antibody arrays datashowed that testosterone induced fast phosphorylation of Akt and MEK/ Erk. Western blot analysis agreed with the arraydata,and it also showed that the activation of the signaling pathways was mediated by AR. We concluded that theactivation of Akt and MEK/ Erk phosphorylation in mouse Sertoli cells was induced by testosterone.