青藤碱对胶原诱导性关节炎小鼠肿瘤坏死因子-α、白介素-17及核因子-κB的影响

Effects of Sinomenine on TNF-α,IL-17 and NF-κB in CIA mice

目的观察青藤碱(SIN)对胶原诱导性关节炎(CIA)小鼠关节炎指数(AI)评分、血清肿瘤坏死因子-α(TNF-α)、白细胞介素-17(IL-17)及滑膜核因子-κB(NF-κB)蛋白表达的影响。方法将30只DBA/1小鼠用随机数字表法分为正常组、CIA组、SIN组3组,每组各10只,诱导CIA组与SIN组小鼠建立CIA模型,予青藤碱(SIN)药物干预SIN组,对三组分别进行AI评分,测定血清TNF-α、IL-17的水平及滑膜NF-κB蛋白的表达。结果 SIN组和CIA组TNF-α、IL-17的水平及滑膜NF-κB蛋白的表达均较正常组升高,且SIN组小鼠的AI评分较CIA组明显降低,血清TNF-α[(170.73±11.05)pg·m L-1比(347.97±13.45)pg·m L-1,F=1 021.67,P<0.01]、IL-17[(470.30±122.02)pg·m L-1比(621.78±48.96)pg·m L-1,F=184.42,P<0.01]水平明显减少,滑膜NF-κB[(0.53±0.04)pg·m L-1比(0.84±0.04)pg·m L-1,F=804.05,P<0.01]蛋白的表达下调。结论 SIN能改善CIA小鼠关节肿胀,降低血清TNF-α、IL-17的分泌,这可能与其下调了滑膜NF-κB的表达有关。

Objective To explore the effect of Sinomenine （SIN） on arthritis index （AI） scores,the serum level of tumor necrosis fac-tor-α（TNF-α）,Interleukin-17 （IL-17） and the protein expression of synovium nuclear factor -κB （NF-κB） in collagen-induced arthri-tis （CIA） mice.Methods The mice were assigned into the normal group ,the CIA group and the SIN group by using a random number table method,ten mice in each group.CIA group and SIN group were induced to establish a CIA model ,and the mice in the SIN group were given Sinomenine drug intervention .The AI scores,the levels of serum TNF-α,IL-17 and synovium NF-κB of the three groups were measured.Results In group SIN and group CIA,the levels of TNF-α,IL-17 and the expression of NF-κB in synovium were higher than those in normal group.The AI scores and the level of TNF-α[（170.73 ±11.05） pg· mL-1 vs （347.97 ±13.45） pg· mL-1 ,F =1021.67,P 〈0.01）],IL-17 [（470.30 ±122.02） pg· mL-1 vs （621.78 ±48.96） pg· mL-1 ,F =184.42,P 〈0.01）] and synovi-um NF-κB [（0.53 ±0.04） pg· mL -1 vs （0.84 ±0.04） pg· mL -1 ,F =804.05,P 〈0.01] in SIN group were significantly decreased compared to those of CIA group.Conclusions SIN can improve the joint swelling of CIA mice and reduce the secretion of serum TNF -α,IL-17,which may be related to downregulating the protein expression of synovium NF -κB.