AMPK在七氟烷致H4细胞凋亡中的作用研究

The Role of AMPK in the Apoptosis of H4 Cells Induced by Sevoflurane

目的:探讨AMPK是否会影响七氟烷引起的细胞凋亡,为研究吸入麻醉药致神经毒性的机制提供新的靶点和思路。方法:用4.1%七氟烷处理H4人脑神经胶质瘤细胞6 h,建立七氟烷细胞毒性模型。建模前予以AMPK激动剂(二甲双胍)或AMPK抑制剂(Compound C)进行预处理。运用Westernblot检测AMPK、p AMPK、full-length caspase-3以及caspase-3 fragment的表达情况。结果:在七氟烷致H4细胞凋亡的模型中,通过AMPK激动剂进一步增加七氟烷所致的p AMPK上调,能够减少细胞凋亡。结论:在七氟烷致H4细胞凋亡的模型中,激活AMPK具有细胞保护作用。

Objective： To investigate whether AMPK can affect the apoptosis induced by sevoflurane and provide new targets and ideas for the study of neurotoxicity induced by inhaled anesthetics. Methods： H4 human neuroglioma cells were treated with 4.1% sevoflurane for 6 hours, and the cytotoxic model of sevoflurne was established. Pre - modeling was pretreated with AMPK agonists （metformin） or AMPK inhibitors （Compound C ）. Western blot is used to detect the expression of AMPK, pAMPK, full-length Caspase-3, and caspase-3 fragment. Results ： In the model of scvoflurane inducing apoptosis in H4 cells, the increase of pAMPK induced by sevoflurane was further increased by AMPK agonists, which could reduce apoptosis. Conclusion ： In the model of sevoflurane inducing apoptosis in H4 cells, the activation of AMPK has the protective effect of cells.