Wnt11和BCL2A1在完全性葡萄胎中的表达及临床意义

Expression of Wnt11 and BCL2A1 in Complete Hydatidiform Mole and its Clinical significance

目的:分析分泌型糖蛋白11(Wnt11)和B淋巴细胞瘤2相关蛋白A1(BCL2A1)在完全性葡萄胎(CHM)中的表达及临床意义。方法:本研究选取了2014年10月~2015年10月于院内接受治疗的80例葡萄胎患者首次清宫所得到的CHM组织作为研究组,同时选取2016年10月~2017年10月于院内进行人工流产的50例患者的绒毛组织作为对照组,使用免疫组化法对组织中的Wnt11和BCL2A1蛋白的表达进行检测,分析CHM组织中的Wnt11和BCL2A1蛋白的表达价值。结果:两组患者在中间型滋养细胞、合体滋养细胞和细胞滋养细胞中的Wnt11 IRS积分以及BCL2A1的DAB百分比的比较中,差异有统计学意义(P<0.05),但在绒毛间质细胞Wnt11的IRS积分比较中无显著差异(P>0.05);当Wnt11和BCL2A1的DAB百分比截断值分别为23.19%和16.33%时,其预测价值达到了最高值;Wnt11和BCL2A1在研究组与对照组之间的表达呈正相关P<0.05)。结论:Wnt11和BCL2A1均可以在正常绒毛和葡萄胎组织中得到表达,且可以有效实现对葡萄胎恶变的预测,宜加强临床中对Wnt11和BCL2A1和合理检测。

Objective： To analyze the wingless 11 （Wntl 1 ） and B cell lymphoma 2 related A1 （BCL2A1） in complete hydatidiform mole （CHM） and its clinical significance. Methods： This study selected the CHM tissue from 80 patients with hydatidiform mole who had been treated in the hospital from October 2014 to October 2015 as the study group, and selected from October 2016 to October 2017 in the hospital 50 cases of induced abortion were used as control group. The expression of Wntll and BCL2AI protein in tissues was detected by immunohistoehemistry, and the expression of Wntl 1 and BCL2A1 protein in CHM tissues was analyzed. Results： The differences in Wntl 1 immunoreaetivity scores and percentage of DAB in BCL2A1 between the two groups were statistically significant （P 〈 0.05 ） in mesenchymal cells, syncytiotrophoblasts and cytotrophoblasts, but not in villus mesenchymal cells Wntl 1 and BCL2A1 had the highest predictive value when the cut-off values of percentage of DAB of Wnt 11 and BCL2A1 were 23.19% and 16.33%, respectively. Wntl 1 and BCL2A1 were significantly higher in the study group and the control group There was a positive correlation between the expression of P 〈0.05 ）. Conclusion ： Both Wnt 11 and BCL2A 1 can be expressed in normal villi and hydatidiform mole, and can effectively predict the malignancy of hydatidiform mole. It is advisable to strengthen the detection of Wntl 1 and BCL2A1 in clinic.