职业铝接触工人肿瘤坏死因子受体1表达与认知功能的关系

The study of the effects of occupational aluminum exposure on TNFR1 expression and cognitive function

目的分析职业铝接触工人血液肿瘤坏死因子受体1（TNFR1）表达与认知功能的关系。方法收集2016年山西某大型铝厂140例工人作为研究对象，其中70名电解铝车间工人为接触组，70名非电解铝车间工人为对照组。用简易精神状态量表（MMSE）、数字广度测试（DST）、物体记忆测验（FOME）和简单反应时测验（SRTT）评价研究对象的认知水平；用石墨炉原子吸收法测定研究对象血浆铝水平作为工人铝接触的内暴露指标；采用RT-PCR法检测其肿瘤坏死因子受体1（TNFR1）mRNA表达水平；用ELISA法测定血浆肿瘤坏死因子受体1（TNFR1）蛋白表达水平。比较各组认知水平和TNFR1表达水平之间的差异。结果接触组血铝含量[（77．12±27．18）μg/L]高于对照组[（55．62±28．69）μg／L]，差异有统计学意义（P〈0．01）。与对照组比较，接触组工人MMSE和FOME得分明显降低，SRTT得分明显升高，差异有统计学意义（P〈0．01）。与对照组比较，接触组TNFR1mRNA相对表达量和TNFR1蛋白表达量均明显升高，差异有统计学意义（P〈0．01）。经相关性分析，接触组中血铝含量与MMSE、DST呈负相关（rMMSE=-0．284，rDST=-0．331，P〈0．05），与SRTF、TNFR1（mRNA）、TNFR1（蛋白）表达量呈正相关（rSRTT=0．255，rmRNA=0．333，r蛋白=0．987，P〈0．01）。MMSE与TNFR1（mRNA）、TNFR1（蛋白）呈负相关（rmRNA=-0．268，r蛋白=-0．255，P〈0．05）。SRTF与TNFR1（蛋白）呈正相关（r蛋自=0．243，P〈0．05）。结论职业铝接触工人认知功能改变与TNFR1表达增加有关。

Objective To analyze the relationship between cognitive function and tumor necrosis factor receptor 1 （TNFR1） expression of occupational exposed workers to aluminum and provide the basis for the diagnosis of cognitive impairment. Methods 140 cases Shanxi aluminum plant workers were collected in 2016 as the research object,including 70 potroom workers for exposure group, 70 non-electrolytic aluminum plant workers in the control group, respectively. Using mini mental status examination （MMSE）, digit span test （DST）, fuld object memory examination （FOME） and simple reaction time test （SRT＇F） evaluate the cognitive function of objects. Using graphite furnace atomic absorption method for the determination of plasma aluminum levels as an indicator of aluminum contact exposure of workers. Using RT-PCR method for detection of tumor necrosis factor receptor 1 （TNFR1） mRNA expression levels. And comparison group differences in cognitive and TNFR1 mRNA expression levels. Results The plasma aluminum content of exposed group （77.12±27.18） μg/L higher than the control group （55.6±28.69） μg/L （P=0.000）; Compared to control group, FOME and MMSE score was significantly increased in the exposed group （P=0.000, P=0.000）, SRTT scores significantly higher in the exposed group （P=0.001）, DST no significant difference in the control group （P=0.893）. Compared to control group, The mRNA expression of TNFR1 was significantly higher in the exposed group （P= 0.002） ; Compared to control group, The protein expression of TNFR1 was significantly higher in the exposed group （P=0.002）. By correlation analysis in exposure group, plasma aluminum content was negatively correlated with MMSE and the DST （r=-0.284, r=-0.331, P〈0.05） and positively correlated with the SRTF, TNFR1 （mRNA） and TNFR1 （protein） （r=0.255, r=0.333, r=0.987, P〈0.01 ）, MMSE was negatively related to TNFR1 （mRNA） and TNFR1 （protein） （r=-0.268, r=-0.255, P〈0.05） ; DST was negatively correlated with the SRTY and TNFR1 （protein） （r=-0.267, r=-0.330, P〈0.05）; SRTF was positively correlated with TNFR1 （protein） （r=0.243, P〈0.05） ; TNFR1 （mRNA） was positively correlated with TNFR1 （protein） （r=0.340, P〈0.01）. Conclusion Cognitive function change of occupational exposed workers to aluminum was related to the increase of TNFR1 expression.