黄秋葵果胶与半乳糖凝集素-3相互作用的研究

The interaction between the pectin and galectin-3 in Abelmoschus esculentus Moench

采用DEAE-纤维素柱层析对黄秋葵果胶进行分离纯化,得到不同结构特征的果胶组分,采用1-苯基-3-甲基-5-吡唑啉酮柱前衍生化HPLC法和HPGPC对分离纯化的果胶组分进行单糖组成分析和分子量分布进行分析,采用半乳糖凝集素-3诱导的红细胞凝集实验和竞争性荧光酶联免疫法研究不同结构特征的黄秋葵果胶组分抑制半乳糖凝集素-3活性的情况。结果表明,黄秋葵果胶经DEAE-纤维素柱层析分级后得到AEP-1和AEP-2 2个产物,均主要由鼠李糖、半乳糖醛酸和半乳糖构成,分子量分别约为380和130 k Da;半乳糖凝集素-3诱导的红细胞凝集实验和竞争性荧光酶联免疫法的实验结果均表明,AEP-2具有较强的抑制半乳糖凝集素-3的活性,初步阐释了黄秋葵抗肿瘤活性的机理,将促进黄秋葵深度开发利用。

The pectin was isolated from Abelmoschus esculentus and purified using DEAE-cellulose column. The monosaccharide composition and molecular weight distribution of the fractions were determined by HPLC with pre-column derivatization using 1-phenyl-3-methyl-5-pyrazolone and HPGPC, respectively. The Gal-3-mediated hemagglutination and competitive fluorescence-linked immunosorbent methods were used to evaluate the interaction between the sub-fractions and Gal-3. Sub-fractions AEP-1 and AEP-2 were obtained using the method of separation and purification, which mainly consisted of rhamnose, galacturonic acid and galactose. The molecular weight of AEP-1 and AEP-2 was 380 and 130 k Da, respectively. The Gal-3-mediated hemagglutination and competitive fluorescence-linked immunosorbent assay results showed that the inhibitory effect of AEP-2 was the highest. This isolation and purification method could be applied to obtain the bioactivity fractions from Abelmoschus esculentus Moench.