山药协同替加氟对结肠癌荷瘤裸鼠免疫功能的影响

Effect of synergism of Chinese yam with tegafur on immunological function in nude mice of colon cancer

目的研究山药提取物联合替加氟对结肠癌的体内抑制作用及免疫功能的影响。方法建立荷结肠癌HT29细胞裸鼠模型,用替加氟(36mg/kg)、山药提取物(125mg/kg)、山药提取物(125mg/kg)协同替加氟(36mg/kg)分别治疗,观察各组抑瘤情况以及血清中的CD4+T和CD8+T比值及白细胞介素-2(IL-2)、α-肿瘤坏死因子(TNF-α)和γ-干扰素(IFN-γ)的含量。结果荷瘤鼠各组治疗结束后,协同组的瘤质量明显低于单独使用山药提取物组及替加氟组,血清中的CD4^+T和CD8^+T比例值及IL-2、TNF-α和IFN-γ的含量明显高于单独使用山药提取物组及替加氟组。结论山药提取物协同替加氟可以提高荷结肠癌裸鼠的免疫力,具有一定的治疗效果。

[Objective]To study the inhibition and immune function effects of co-administration of tegafu and Chinese yam on colon cancer. [Methods] The nude mice model of colon cancer HT29 cells was established. The treatment was given in using tegafur（36mg/kg）,Chinese yam extract（125mg/kg）,and combined utilization method. The tumor suppressor situation was observed,and serum CD4＋T/CD8＋T,contents of interleukin-2（IL-2）,α-tumor cell necrosis factor（TNF-α） and γ-interferon（IFN-γ）were calculated. [Results] After the treatment,the tumor quality of the combined treatment was lower than that of Chinese yam group and tegafur group,while serum CD4-＋T/CD8-＋T and contents of IL-2,TNF-α and IFN-γ were significantly higher than those in the two isolated groups. [Conclusion] The synergistic combination of the Chinese yam extract and the tegafur can improve the immunity of the nude mice with colon cancer and shows certain therapeutic effect.