摘要
目的调查中国羊种布鲁氏菌rpoB基因的多态性。方法利用AMOS-PCR对分离菌株进行种型鉴定;根据布鲁氏菌rpoB基因的4个多态性位点设计特异性引物,对299株羊种布鲁氏菌流行株(羊1型菌38株、羊2型菌19株、羊3型菌242株)的rpoB基因的4个多态性位点进行扩增,并将PCR产物测序结果与羊种布鲁氏菌标准菌株的扩增结果进行比对。结果羊种布鲁氏菌的rpoB基因检测发现,有169株为rpoB-2变异型、103株为rpoB-2型、24株为新的rpoB基因型(rpoB-4型)及3株为rpoB-1型。结论我国羊种布鲁氏菌rpoB基因具有较高的多态性,可作为羊种布鲁氏菌的分子流行病学调查靶基因,我国羊种菌rpoB基因型和生物型无相关性。
ObjectiveTo investigate polymorphism of rpoB gene of Brucella melitensis strains isolated in China.Methods Species/biovars ofB.melitensisstranis were identified by AMOS-PCR. Then,specific primers were designed according to fourpolymorphic loci ofBrucella rpoBgene,four diversity loci of rpoB gene of 299 B.melitensis stranis[including B. melitensisbiovar 1（38）,B. melitensisbiovar 2（19）,B. melitensisbiovar 3（242）]were amplified,and sequencing results of PCRproduct were compared with rpoB gene of standard strains of B. melitensis.ResultsAnalysis of rpoB gene of 299 B. melitensisstrains revealed that 169 strains were rpoB-2 variants genotype,103 were rpoB-2 genotype,24 strains exhibited new rpoBgenotype（named asrpoB-4）,3 strains of rpoB-1 genotype.Conclusion Rpo Bgene ofB. melitensisfrom China has highergenetic polymorphism,rpoBgene can be used as a target gene for molecular epidemiological investigation ofB. melitensis.
作者
李明慧
刘志国
朴东日
赵鸿雁
田国忠
姜海
Li Minghui;Liu Zhiguo;Piao Dongri;Zhao Hongyan;Tian Guozhong;Jiang Hai(State Key Laboratory for Infectious Dtsease Prevention ana Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;Ulanqab Centre for Endemic Disease Control and Prevention, Ulanqab 012000, Inner Mongolia, China)
出处
《疾病监测》
CAS
2018年第3期193-197,共5页
Disease Surveillance
