摘要
目的:探讨乙肝病毒X区基因(hepatitis B virus X gene,HBV-X)变异情况与慢性乙肝病毒(hepatitis B virus,HBV)感染患者血清中转化生长因子-β1(transforming growth factor beta 1,TGF-β1)表达致肝细胞肝癌间的相关性。方法:收集2015年1月至2016年10月期间重庆医科大学附属第二医院的慢性乙型病毒性肝炎患者血清35例,肝硬化35例,肝癌35例。采用聚合酶链反应方法(polymerase chain reaction,PCR)扩增HBV-X基因序列,并对其产物行DNA测序,将测序结果与已知HBV-X基因序列对比分析变异位点。酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA)检测患者血清中TGF-β1浓度。将结果进行卡方检验、单因素方差分析等方法处理数据。结果:TGF-β1浓度在肝癌组表达明显高于非肝癌组,差异具有统计学意义(P<0.05)。肝病患者血清中常见的突变位点依次为:A1762T/G1764A、T1719C、G1635A、A1605G、C1653T、T1753G,其中A1605G、A1762T/G1764A、T1753G突变率在肝癌组明显高于非肝癌组,差异具有统计学意义(P<0.05)。采用t检验对两者进行分析发现,3个位点突变与不突变组的TGF-β1浓度存在统计学差异。结论:HBV-X基因中的T1753G、A1605G、A1762T/G1764A位点突变可能与TGF-β1表达及肝癌的发生相关,这可能参与了肝癌疾病发展进程。
Objective:To study the correlation between hepatitis B virus X gene(HBV-X gene)mutation and transforming growth factor beta 1(TGF-β1)in hepatocarcinogenesis among the patients with chronic hepatits B virus(HBV)infection. Methods:The serum samples of 105 patients with HBV infected were collected in The Second Affiliated Hospital of Chongqing Medical University from2015 to 2016. They were divided into chronic hepatits B group(35 cases),liver cirrhosis groups(35 cases),primary liver cancer group(35 cases). The HBV-X gene sequences were amplified from the serum samples by polymerase chain reaction(PCR),then the amplified products were sequenced,finally compared with those reported in Gen Bank to find the variable sites. The levels of TGF-β1 from serum samples were detected by enzyme-linked immunosorbent assay. Results:The level of TGF-β1 was significantly higher in primary liver cancer group than in other groups. The common HBV-X gene mutation sites including :A1762 T/G1764 A,T1719 C,G1635 A,A1605 G,C1653 T,T1753 G. The HBV-X gene mutation including A1605 G,A1762 T/G1764 A,T1753 G was significantly higher in primary liver cancer group than in other groups. T test was sued to compare HBV-X gene mutation sites and the level of TGF-β1 in all patients;there were statistical differences in TGF-β1 levels of three mutation sites between mutation group and non-mutation group. Conclusion:The HBV-X gene mutation sites including A1605 G,A1762 T/G1764 A,T1753 G may have correlation with the level of TGF-β1 and HCC,which may participate in the occurrence of hepatocellular carcinoma.
作者
王玉凤
何松
Wang Yufeng;He Song(Department of Gastroenterolog;The Second Affiliated Hospital of Chongqing Medical Universit)
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2018年第5期646-650,共5页
Journal of Chongqing Medical University
基金
重庆市卫生局医学科研计划重点资助项目(编号:2013-1-019)
关键词
乙肝病毒X基因
点突变
肝细胞肝癌
转化生长因子-Β1
hepatitis B virus X gene
point mutation
hepatocellular carcinoma
transforming growth factor β1