肤敏膏对机械性损伤创面愈合和瘢痕形成的影响

Effect of Fumin Gao on Healing of Skin Wound and Scar Formation Caused by Mechanical Trauma

目的:观察中药复方肤敏膏对机械性损伤致创面愈合和瘢痕形成的影响。方法:实验动物随机分为阳性对照组、模型组、肤敏膏高剂量组、肤敏膏低剂量组,以家兔耳部皮肤全层切除(深度至软骨,面积为1.0 cm×0.8 cm)机械伤为模型。观察术后创面愈合时间,于术后2 d、3 d、5 d、7 d测定伤腔容积,术后2 d、4 d、6 d测定伤口红肿宽度,术后7 d、14 d、28 d、56 d切取兔耳皮肤全层组织,测量毛细血管数、表皮生长宽度、肉芽组织厚度、上皮生长宽度、纤维细胞层厚度。结果:与模型组比较,肤敏膏高剂量、低剂量均可缩短创面愈合时间,其中高剂量肤敏膏可明显缩短创伤愈合时间(P<0.05);肤敏膏高剂量、低剂量均能显著缩小兔耳部皮肤全层切除伤伤腔容积(P<0.05),缓减创面边缘的红肿程度,显著缩小创伤后创面红肿宽度(P<0.05),促进全层切除上皮的生长,促进家兔耳部皮肤全层切除伤肉芽组织的生长,提高创面愈合率;肤敏膏高剂量、低剂量均能防止毛细血管减少(P<0.05),减缓纤维细胞层增厚(P<0.05),抑制兔耳部皮肤全层切除伤表皮增厚。结论:肤敏膏在创伤愈合阶段可明显促进创面愈合,在瘢痕形成阶段可缓减胶原纤维过度增生。

Objective： To observe the effect of Fufang Fumin Gao on wound healing and scar formation caused by mechanical damage. Methods： The experimental animals were randomly divided into positive control group,model group,high dose Fufang Fumin Gao group,low dose Fufang Fumin Gao group. The ear injury of rabbits was made by full-thickness resection of the ear skin(depth to cartilage area 1. 0 cm × 0. 8 cm）. The concept of time check wound healing after surgery,postoperative 2 d,3 d,5 d,7 d determination of wound cavity volume,postoperative 2 d,4 d,6 d determination of wound swelling width,postoperative 7 d,14 d,28 d,56 d tissue cutting and rabbit ear skin,counting the capillary number,width,epidermal growth of granulation tissue the thickness of skin growth width,fiber cell layer thickness. Results： Fufang Fumin Gao high dose and low dose can shorten wound healing time,the high dose group can significantly shorten the healing time of sensitive skin cream; high dose and low dose can significantly reduce the rabbit ear skin full-thickness excision wound cavity volume,slow swelling degree reduction of wound edge,reduce wound swelling after trauma area,reduce swelling height,promote full-thickness resection of epithelial growth,promote rabbit ear skin fullthickness excision wound granulation tissue growth,improve the rate of wound healing; skin cream sensitive high dose and low dose groups could prevent capillary reduction,slow fiber cell layer thickening,inhibition of rabbit ear skin full-thickness excision wound epidermal thickening. Conclusion： Fufang Fumin Gao can obviously promote wound healing in the stage of wound healing,and it can reduce the hyperproliferation of collagen fibers in the stage of scar formation.