亳菊中6种黄酮类化合物的分离及其对心肌细胞H9c2的保护作用

Isolation of Six Flavonoids from Boju and Their Protection Role on Cardiomyocytes

目的:研究亳菊中主要黄酮类化合物对心肌细胞增殖的影响,筛选出亳菊中起心肌细胞保护作用的主要黄酮类化合物。方法:采用传统提取分离方法从亳菊中提取总黄酮,并利用多种柱色谱方法分离得到亳菊中主要的黄酮类化合物,通过光谱分析鉴定其结构。采用MTT法分析评价亳菊中主要的黄酮类化合物促进大鼠心肌细胞H9c2增殖的作用。结果:在亳菊中分离得到金合欢素、芹菜素、木犀草素、金合欢素-7-O-β-D-葡萄糖苷、芹菜素-7-O-β-D-葡萄糖苷、木犀草素-7-O-β-D-葡萄糖苷,6种黄酮类化合物。与正常对照组比较,除芹菜素-7-O-β-D-葡萄糖苷外,其余各黄酮类化合物均显示出对心肌细胞增殖的促进作用,其中木犀草素组和金合欢素-7-O-β-D-葡萄糖苷组OD值明显升高,且与对照组比较,有显著性差异(P<0.05)。结论:木犀草素、金合欢素-7-O-β-D-葡萄糖苷具有显著的促进心肌细胞H9c2增殖的作用。

Objective： To screen the main flavonoids which are in charge of myocardial cell protecting by studying the effects of some flavonoids in Boju on myocardial cell proliferation. Methods： Traditional technologies for extracting and isolating were adopted to yield total flavonoids. Then various column chromatographies were used in isolating and purifying for the main flavonoids in Boju,the chemical structures of which were identified with spectroscopic analysis. MTT assay was adopted to evaluate the capability of flavonoids in Boju on promoting myocardial cell H9 c2 proliferation from rats. Results： Six flavonoids were isolated from Boju as Acacetin,Apigenin,Luteolin,Acacetin-7-O-β-D-glucoside,Apigenin-7-O-β-D-glucoside,and Luteolin-7-O-β-D-glucoside. Compared with the normal control group,flavonoids exhibited positive effects on myocardial cell proliferation except Apigenin-7-O-β-Dglucoside. Furthermore,the OD value of Luteolin group and Acacetin-7-O-β-D-glucoside group raised evidently,and showed significant difference with the normal control group(P 〈 0. 05）. Conclusion： Luteolin and Acacetin-7-O-β-D-glucoside show positive effects on myocardial cell H9 c2 proliferation.