柚皮素对凝血测试结果的影响

Effect of naringenin on coagulation test

目的:探究柚皮素对凝血功能测定过程的影响,及柚皮素在凝血诊断测试中对二价金属离子的屏蔽作用,为柚皮素在凝血诊断试剂中的应用提供理论依据。方法:分别配置不同浓度梯度的柚皮素乳浊液,加入定量标准血浆,Sysmex 1500全自动凝血分析仪分析对凝血功能检测结果的影响。每个试样测试4次,同时在添加柚皮素的前提下,在待测血浆中加入二价金属离子Cu^(2+)和Zn^(2+),测试对凝血功能检测结果的影响。结果:柚皮素的浓度低于1.5×10^(-5) mol/L时,对APTT、PT和TT无影响。柚皮素为(0.5~4.5)×10^(-4) mol/L时,检测值差异无统计学意义(P>0.05),随着柚皮素加入量的增加,APTT呈增加的趋势。在待测血浆中加入柚皮素的前提下,添加二价金属离子Cu^(2+)和Zn^(2+)与未加入上述2种离子相比,凝血测试结果差异无统计学意义(P>0.05)。结论:(0.5~4.5)×10^(-4) mol/L范围内柚皮素的加入对凝血功能检测结果差异无统计学意义,同时具有螯合二价金属离子的作用,从而屏蔽二价金属离子对凝血因子的抑制。

Objective：To investigate the significance of naringenin on blood coagulation test in order to provide theoretical basis for the application of naringenin in coagulation diagnostic reagents.Method：Low concentration and high concentration naringenin were preparedseparately with different concentration gradients,then added quantitative standard plasma and tested by Sysmex 1500 Automatic Coagulation Analyzer.Each specimen was tested 4 times.Result：It showed that there was no effect on APTT,PT and TT when the amount of naringenin was below1.5×10^-5 mol/L.When the addition of naringenin was 5×10^-5 to 4.5×10^-4 mol/L,there was no significant difference in the detection values（P〉0.05）,but with the increase of naringenin added,APTT showed an increasing trend.With naringenin added to the plasma,both Cu^2＋ and Zn^2＋ in the plasm showed no statistical significance for coagulation tests（P〉0.05）.Conclusion：The appropriate dose of naringin would have no effect on the detection result of coagulation function and havethe effect of chelating heavy metal ions,thus reducing the inhibition of Cu^2＋ and Zn^2＋ on coagulation factors.