LncRNA-7460调控炎症来源牙周膜干细胞成骨分化的研究

Long non-coding RNA 7460 regulates the osteogenic differentiation of h PDLSCs

目的:探讨长链非编码RNA(lncRNA)在牙周膜干细胞成骨分化过程中的调控作用。方法:运用实时荧光定量聚合酶链反应实验(q PCR)观察表达量在成骨分化前后发生明显变化的lncRNA,筛选得到成骨相关的lncRNA-7460。运用慢病毒分别上调、下调炎症来源牙周膜间充质干细胞(PPDLSCs)内的lncRNA-7460,q PCR检测成骨相关基因mRNA的变化,用碱性磷酸酶(ALP)染色、ALP活性测定、茜素红染色等方法验证PPLDSCs成骨状态。数据进行统计学处理。结果:在PPDLSCs中上调lncRNA-7460引起成骨相关基因的表达上升,ALP活性增高,形成更多的钙结节;下调lncRNA-7460导致成骨相关基因表达下降,细胞ALP活性和成骨结节形成能力降低。结论:lncRNA-7460可以在体外培养中促进PPDLSCs的成骨分化能力。

Objective： To investigate the regulation of Long none noding RNAs （lncRNAs） in the osteogenic differentiation of human periodontal ligament stem cells （hPDLSCs）. Methods： Real-time quantitative PC R （qPCR） was used to observe the different expression of lncRNA in hPDLSCs before and after osteogenic differentiation. And then, osteogenic-related lncRNA, lncRNA-7460 was screened out. After using lentivirus to overexpress and knockdown lncRNA-7460 in hPDLSCs respectively, qPCR was used to detect the mRNA expression of osteogenesis related genes. ALP activity assay and alizarin red staining were used to verify the effect of lncRNA-7460 on PPDLSCs. Data were statistically processed. Results： Overexpressing lncRNA-7460 in PPDLSCs promoted the expression of osteogenic differentiation related genes, increased ALP activity and mineralized nodule formation. Downexpression of lncRNA- 7460 downregulated the expression of osteogenic differentiation related genes, decreased ALP activity and mineralized nodule formation. Conclusion： LncRNA-7460 can promote osteogenic differentiation of PPDLSCs in vitro.