利用耐盐菌以餐厨垃圾为碳源合成PHA的条件优化的研究

Optimization of Conditions for PHA Synthesis from Salt Tolerant Bacteria Using Kitchen Waste as Carbon Source

PHA是由微生物(主要是细菌)在细胞内合成的脂类物质,具有合成塑料的物化特性且具备可生物降解性,是天然的高分子材料,可以替代传统塑料。利用餐厨垃圾作为碳源,可以降低合成PHA的生产成本,同时对餐厨垃圾的资源化利用具有重要的意义。含盐量高是我国餐厨垃圾的显著特征,因此本研究从含盐量高的生活垃圾渗沥液处理系统和盐碱地中筛选分离出PHA合成能力较强的3种菌,经过16S rDNA分析确定其菌种,分别是:三种菌株分别为苏云金芽孢杆菌菌株IARI-UPS 6,苏云金芽孢杆菌菌株L14,蜡样芽胞杆菌菌株FORC_024。同时探究了不同接种率、pH值及餐厨废油添加量对3种菌合成PHA能力的影响。

Polyhydroxyalkanoates（PHA）is a kind of polymer bioopolymer synthetized inside the cell by microorganism. It not only has the physicochemical properties of synthetic plastics, but also has biodegradability. It can replace traditional plastics. The use of kitchen waste as a carbon source for the synthesis of PHA can reduce the cost of production, and it is of great significance to the utilization of food waste in the resource utilization.The high salt content is a significant feature of kitchen waste in our country. Therefore, 3 kinds of salt-tolerant strains with synthetic PHA function were isolated from the leachate treatment system and saline-alkali soil. After 16S rDNA analysis to determine the bacteria.,Tthe results showed that the three strains were Bacillus thuringiensis strain IARI-UPS 6, Bacillus thuringiensis strain L14, Bacillus cereus strain FORC024.This study also researched the influence of diverse inoculation rate, pH, and amount of kitchen waste oil on the ability of these three kinds of bacteria to synthetize PHA.