近红外光谱技术快速测定黄连女贞子提取过程中2种成分含量

Rapid Determination of Two Index Components in Extracting Process of Fructus Ligustri Lucidi and Coptis by Near-Infrared Spectroscopy

目的:建立一种用近红外光谱技术快速测定女贞子黄连提取过程指标成分含量的方法。方法:运用偏最小二乘法结合多种光谱预处理方法及波长选择方法建立近红外光谱与女贞子黄连提取液指标成分(盐酸小檗碱、盐酸巴马汀)含量之间校正模型,通过交互检验标准偏、校正标准偏差、决定系数和主因子数优选校正模型,并对未知样本进行预测分析。结果:一提液中盐酸小檗碱、盐酸巴马汀的校正模型相关系数分别为99.46、98.30,二提液中三盐酸小檗碱、盐酸巴马汀的校正模型相关系数分别为99.16、97.86。验证集的预测值与真实值含量接近。结论:该方法操作简便、快速无损、准确可靠,可用于女贞子黄连提取过程指标成分含量的快速监测。

Objective： To establish a near-infrared（NIR） rapid quantitative analysis method of the index components（berberine hydrochloride and palmatine hydrochloride） of the extracting process of Fufang Zhenzhu Tiaozhi formula（ FTZ）. Method： All samples obtained from the processes of extractions were analyzed by NIR spectra and HPLC. To establish the calibration model, the THUNIR software was used to combine the data of NIR and HPLC with different pretreatment methods, band selection methods and partial least square method. The standard error of cross-validation, standard error of calibration, determination coefficient and latent variables were used to optimize the quantitative analysis models. Result： The established models of 1 st extraction and 2 nd extraction of berberine hydrochloride and palmatine hydrochloride showed that the determination coefficient was 99.46, 98.30, 99.16 and 97.86. Tests of the optimal models with prediction samples were made, which showed that the relative deviation of 1 st extraction and 2 nd extraction of these two components were closed. Conclusion： Therefore, NIR technique can be used in determination of berberine hydrochloride and palmatine hydrochloride in extraction processes and it provide a rapid, simple, accurate and on-line detection method for monitoring current extracting process of the extracting process of FTZ.